Alpha-Crystallin Promotes Rat Axonal Regeneration Through Regulation of RhoA/Rock/Cofilin/MLC Signaling Pathways
| Autor: | Nan Wu, Zheng Qin Yin, Dong Wu Wang, Yan Hua Wang, Yi Wang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Cofilin 1
Myosin Light Chains Myosin light-chain kinase RHOA Neurite medicine.medical_treatment Cellular and Molecular Neuroscience Myelin Optic Nerve Diseases medicine Animals Rats Long-Evans alpha-Crystallins Cells Cultured Myelin Sheath rho-Associated Kinases biology Optic Nerve General Medicine Cofilin Molecular biology Axons eye diseases Nerve Regeneration Rats Cell biology medicine.anatomical_structure Animals Newborn nervous system biology.protein Phosphorylation sense organs Signal transduction Axotomy rhoA GTP-Binding Protein Signal Transduction |
| Zdroj: | Journal of Molecular Neuroscience. 46:138-144 |
| ISSN: | 1559-1166 0895-8696 |
| DOI: | 10.1007/s12031-011-9537-z |
| Popis: | Intravitreal injection of α-crystallin can promote axons from optic nerve regeneration after crushing in rats. We have previously demonstrated that α-crystallin can counteract the effect of myelin inhibitory factors and stimulate neurite growth. And a common crucial signaling event for myelin inhibitory factors is the activation of RhoA. To investigate whether α-crystallin counteracts the inhibitory effect of myelin inhibitory factors through regulation of RhoA/Rock signaling pathway, α-crystallin (10(-4) g/L) was injected into rat vitreous at the time the optic nerve crushed. The RhoA protein activity and the expression of RhoA and Rock were evaluated after 3 days of optic nerve axotomy. Rock downstream effectors, phosphorylated cofilin, and phosphorylated myosin light chain were detected when retinal neurons were cultured for 3 days. Axonal regeneration and neurites growth of cultured cells were observed also. Our results showed that α-crystallin decreased the RhoA protein activity and the phosphorylation of both cofilin and myosin light chain, and promoted the axonal growth. However, the expression of RhoA and Rock was not affected by α-crystallin. These findings indicated that α-crystallin could counteract the effect of myelin inhibitory factors through the regulation of RhoA/Rock signaling pathway. |
| Databáze: | OpenAIRE |
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