Induction of Anti-Theileria sergenti Antibodies in Calves with Murine Monoclonal Anti-Idiotype Antibody
| Autor: | Kiyoshi Takahashi, Masayuki Tanaka, Takashi Matsuba, Masaho Ikeda, Norimasa Sasaki, Misao Onuma, Tatsuji Okabe |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
General Veterinary
medicine.diagnostic_test Inoculation Vaccination Antibody titer Antibodies Protozoan Fluorescent Antibody Technique Anti-Idiotype Antibody Enzyme-Linked Immunosorbent Assay Macrophage Activation Biology Theileria sergenti Immunofluorescence Virology Antibodies Anti-Idiotypic Theileriasis Antigen Theileria Monoclonal medicine biology.protein Animals Cattle Antibody |
| Zdroj: | Journal of Veterinary Medical Science. 53:775-778 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.53.775 |
| Popis: | Anti-idiotype (anti-Id) antibody that contains an internal image of Theileria sergenti (T. sergenti) merozoite surface antigen was intramuscularly inoculated to calves to induce anti-T. sergenti antibody. In immunized calves, anti-anti-Id antibodies were induced and these antibodies bound to merozoites of T. sergenti were confirmed by immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). In this experiment, one calf with the highest antibody titer against anti-Id antibody was intravenously challenged with T. sergenti-infected erythrocytes to show insufficient protective effects of the anti-anti-Id antibodies against the challenge. |
| Databáze: | OpenAIRE |
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