Methanosarcina spelaei sp. nov., a methanogenic archaeon isolated from a floating biofilm of a subsurface sulphurous lake
| Autor: | Mashal Alawi, Dirk Wagner, Lars Ganzert, Alexandra Hillebrand-Voiculescu, Wolfgang Sand, Kai Mangelsdorf, Janosch Schirmack |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data ved/biology.organism_classification_rank.species Chemie Trimethylamine Biology Microbiology 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Yeast extract Phylogeny Ecology Evolution Behavior and Systematics Archaeol 030304 developmental biology Base Composition 0303 health sciences Strain (chemistry) Romania 030306 microbiology ved/biology Biofilm Nucleic Acid Hybridization Movile Cave Sequence Analysis DNA General Medicine 16S ribosomal RNA Lipids Caves Lakes DNA Archaeal chemistry Biochemistry Biofilms Methanosarcina Methanosarcina barkeri Water Microbiology Biologie Sulfur |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 64:3478-3484 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A novel methanogenic archaeon, strain MC-15T, was isolated from a floating biofilm on a sulphurous subsurface lake in Movile Cave (Mangalia, Romania). Cells were non-motile sarcina-like cocci with a diameter of 2–4 µm, occurring in aggregates. The strain was able to grow autotrophically on H2/CO2. Additionally, acetate, methanol, monomethylamine, dimethylamine and trimethylamine were utilized, but not formate or dimethyl sulfide. Trypticase peptone and yeast extract were not required for growth. Optimal growth was observed at 33 °C, pH 6.5 and a salt concentration of 0.05 M NaCl. The predominant membrane lipids of MC-15T were archaeol and hydroxyarchaeol phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol as well as hydroxyarchaeol phosphatidylserine and archaeol glycosaminyl phosphatidylinositol. The closely related species, Methanosarcina vacuolata and Methanosarcina horonobensis, had a similar composition of major membrane lipids to strain MC-15T. The 16S rRNA gene sequence of strain MC-15T was similar to those of Methanosarcina vacuolata DSM 1232T (sequence similarity 99.3 %), Methanosarcina horonobensis HB-1T (98.8 %), Methanosarcina barkeri DSM 800T (98.7 %) and Methanosarcina siciliae T4/MT (98.4 %). DNA–DNA hybridization revealed 43.3 % relatedness between strain MC-15T and Methanosarcina vacuolata DSM 1232T. The G+C content of the genomic DNA was 39.0 mol%. Based on physiological, phenotypic and genotypic differences, strain MC-15T represents a novel species of the genus Methanosarcina , for which the name Methanosarcina spelaei sp. nov. is proposed. The type strain is MC-15T ( = DSM 26047T = JCM 18469T). |
| Databáze: | OpenAIRE |
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