Antioxidant, hepatoprotective, genoprotective, and cytoprotective effects of quercetin in a murine model of arthritis
Autor: | Karine Lanes da Silveira, Ivana Beatrice Mânica da Cruz, Fátima Husein Abdalla, Daniela F. Passos, Renata da Silva Pereira Saccol, Emerson André Casali, Cinthia Melazzo de Andrade, Alessandra G. Manzoni, Fernanda Barbisan, Guilherme Lopes Dornelles, Juliana Sorraila de Oliveira, Daniela B.R. Leal |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Freund's Adjuvant Arthritis Pharmacology medicine.disease_cause Biochemistry Thiobarbituric Acid Reactive Substances Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals heterocyclic compounds Lymphocytes Rats Wistar Molecular Biology chemistry.chemical_classification Reactive oxygen species biology Superoxide Dismutase Cell Biology Glutathione medicine.disease Catalase Rats Disease Models Animal Oxidative Stress 030104 developmental biology chemistry Liver 030220 oncology & carcinogenesis biology.protein Female Quercetin Comet Assay Lipid Peroxidation Reactive Oxygen Species Genotoxicity Oxidative stress DNA Damage Mutagens |
Zdroj: | Journal of cellular biochemistryREFERENCES. 121(4) |
ISSN: | 1097-4644 |
Popis: | Rheumatoid arthritis is a highly debilitating inflammatory autoimmune disease which is characterized by joint destruction. The present study sought to investigate the effect of quercetin in rats with complete Freund's adjuvant-induced arthritis. Animals were divided into control/saline, control/quercetin (5 mg/kg, 25 mg/kg, and 50 mg/kg) arthritis/saline, and arthritis/quercetin (5 mg/kg, 25 mg/kg, and 50 mg/kg); the treatments were administered for 45 days. Biochemical, oxidative stress, genotoxicity, and cytotoxicity parameters were evaluated. All doses of quercetin reduced the levels of aspartate aminotransferase, thiobarbituric acid-reactive substances, and reactive oxygen species; however, only treatment with 25 or 50 mg/kg increased catalase activity. Total thiol and reduced glutathione levels were not significantly affected by the induction nor by the treatments. Genotoxicity assessed by DNA damage, and cytotoxicity through picogreen assay, decreased after treatments with quercetin. Our results present evidence of the antioxidant, cytoprotective, genoprotective and hepatoprotective, and effects of quercetin, demonstrating its potential as a candidate for coadjuvant therapy. |
Databáze: | OpenAIRE |
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