Biosynthesis of medium chain length poly(3-hydroxyalkanoates) (mcl PHAs) from cosmetic co-products by Pseudomonas raguenesii sp. nov., isolated from Tetiaroa, French Polynesia

Autor: Gérard Raguénès, Sophie Schmitt, Christelle Gouin, Bernard Costa, Karine Alain, Philippe Crassous, Jean Guezennec, Nelly Kervarec, Christelle Simon-Colin
Přispěvatelé: Centre de Brest, BIOMAR/BMM, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de microbiologie des environnements extrêmophiles (LM2E), Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Service Commun RMN-RPE, Université de Brest (UBO), Centre de Brest, EEP/LME, Pacific Biotech
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Magnetic Resonance Spectroscopy
Cosmetics
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics
Phylogenetics and taxonomy
mcl PHAs
Spectroscopy
Fourier Transform Infrared
Organic chemistry
MESH: Phylogeny
Waste Management and Disposal
Phylogeny
0303 health sciences
biology
Calorimetry
Differential Scanning
Chemistry
Polyhydroxyalkanoates
Pseudomonas
Temperature
MESH: Polynesia
General Medicine
Nuclear magnetic resonance spectroscopy
MESH: Cosmetics
Lipids
MESH: Temperature
MESH: Glass
MESH: Biotechnology
Thermodynamics
MESH: Thermodynamics
Biotechnology
Co-products
Environmental Engineering
Stereochemistry
Bioengineering
Gas Chromatography-Mass Spectrometry
Polynesia
MESH: Spectroscopy
Fourier Transform Infrared
03 medical and health sciences
Differential scanning calorimetry
Microbial mat
MESH: Polyhydroxyalkanoates
Fourier transform infrared spectroscopy
030304 developmental biology
Molecular mass
030306 microbiology
Renewable Energy
Sustainability and the Environment
MESH: Magnetic Resonance Spectroscopy
Enthalpy of fusion
MESH: Calorimetry
Differential Scanning
MESH: Pseudomonas
biology.organism_classification
MESH: Lipids
Culture Media
MESH: Gas Chromatography-Mass Spectrometry
MESH: Culture Media
Gas chromatography
Glass
Zdroj: Bioresource Technology
Bioresource Technology, Elsevier, 2009, 100 (23), pp.6033-6039. ⟨10.1016/j.biortech.2009.06.075⟩
ISSN: 0960-8524
DOI: 10.1016/j.biortech.2009.06.075⟩
Popis: International audience; A new bacterium, designated as strain TE9 was isolated from a microbial mat in French Polynesia and was studied for its ability to synthesize medium chain length poly-beta-hydroxyalkanoates (mcl PHAs) during cultivation on cosmetics co-products. The composition of PHAs was analysed by coupled gas chromatography mass spectroscopy (GC/MS), nuclear magnetic resonance (NMR) and Fourier Transform InfraRed (FTIR) spectroscopy. PHAs were composed of C6-C14 3-hydroxyacids monomers, with a predominance of 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD) and 3-hydroxydodecanoate (3HDD). Differential scanning calorimetry (DSC) experiments allowed the characterization of elastomeric materials with a melting point T(m) near 50 degrees C, enthalpy of fusion DeltaH(m) from 27 to 32 J/g, and glass transition temperature T(g) of -43 degrees C. Molecular weights ranged from 175,000 to 358,000 g/mol. On the basis of the phenotypical features and genotypic investigations, strain TE9 was assigned to the Pseudomonas genus and the name of Pseudomonas raguenesii sp. nov. is proposed.
Databáze: OpenAIRE
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