An automated system for animal exposure to nitrogen dioxide
| Autor: | Vittorio Alongi, Annalaura Erroi, Claudio Zucchi, Paolo Pagani, Maria Romano, Marco Moggi, Barozzi Fabio Michele, Mario Salmona, Ugo Dal Molin, Marina Ferro |
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| Rok vydání: | 1994 |
| Předmět: |
inorganic chemicals
Male Atmosphere Exposure Chambers Neutrophils Nitrogen Dioxide ANIMAL EXPOSURE Toxicology complex mixtures chemistry.chemical_compound Air pollutants medicine Animals Nitrogen dioxide Lymphocytes Lung Pharmacology Chromatography medicine.diagnostic_test Chemistry Macrophages Humidity respiratory system respiratory tract diseases Rats Bronchoalveolar lavage Environmental chemistry Personal computer Current (fluid) Bronchoalveolar Lavage Fluid |
| Zdroj: | Journal of pharmacological and toxicological methods. 31(4) |
| ISSN: | 1056-8719 |
| Popis: | We have designed an automated system for controlled exposure of rodents to nitrogen dioxide (NO2). The system consists of 1) two stainless-steel exposure chambers (3.96 m3), for control and treated animals, in which temperature, humidity, and air changes are consistent with current guidelines for rodent housing; 2) a cylinder containing 1% NO2 in N2; 3) a detector connected with a personal computer system that monitors the NO2 concentration, and regulates the NO2 influx, using a closed-loop feedback; and 4) a fan system to distribute the gas uniformly within the chambers. In a typical experiment rats were exposed to 15 ppm of NO2, and changes in the differential count of polymorphonuclear leukocytes, macrophages, and lymphocytes in bronchoalveolar lavage fluid were investigated. |
| Databáze: | OpenAIRE |
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