A primary transcript in spinach chloroplasts that completely lacks a 5' untranslated leader region
| Autor: | E. M. Orozco, D. C. Bennett, Sharon A. Rogers, Liang-Jwu Chen |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Chloroplasts
Transcription Genetic Molecular Sequence Data Plant Science Biology Primary transcript chemistry.chemical_compound Start codon Transcription (biology) RNA polymerase Genetics Coding region Promoter Regions Genetic Adenosine Triphosphatases Messenger RNA Base Sequence food and beverages RNA General Medicine DNA Plants Molecular biology Ribosomal binding site chemistry Protein Biosynthesis Agronomy and Crop Science Plasmids |
| Zdroj: | Plant molecular biology. 15(1) |
| ISSN: | 0167-4412 |
| Popis: | A fifth, previously undetected transcript for the plastid gene encoding the beta subunit of spinach chloroplast ATPase (atpB) has recently been identified in vitro. In this report we show that this transcript is present in vivo and its 5' end is at the translation start codon. When synthesized in vitro using spinach chloroplast RNA polymerase, the 5' end of this novel transcript is located at the beginning of the second codon of the atpB coding sequence. Although this atpB transcript lacks an untranslated leader region, it is an abundant RNA in vivo and is associated with crude polysomal fractions, as are the four larger atpB transcripts. In vitro synthesis of the leaderless transcript does not occur for a plasmid DNA in which the "-35' region of the promoter is deleted. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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