A proliferation-inducing ligand sustains the proliferation of human naïve (CD27−) B cells and mediates their differentiation into long-lived plasma cells in vitro via transmembrane activator and calcium modulator and cyclophilin ligand interactor and B-cell mature antigen
| Autor: | Masataka Haneda, Yoshiko Matsuda, Kenji Kadomatsu, Takaaki Kobayashi |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Transmembrane Activator and CAML Interactor Protein Tumor Necrosis Factor Ligand Superfamily Member 13 Immunology Naive B cell Cell Culture Techniques Long-lived plasma cell Biology Young Adult Antigen medicine Humans B-Cell Maturation Antigen B cell Cyclophilin B-Lymphocytes Activator (genetics) Antibodies Monoclonal Cell Differentiation Middle Aged Flow Cytometry A proliferation-inducing ligand Molecular biology In vitro Transmembrane activator and calcium modulator and cyclophilin ligand interactor B-cell mature antigen medicine.anatomical_structure Female Tumor necrosis factor alpha Bone marrow Naïve B cell |
| Zdroj: | Cellular Immunology. 295:127-136 |
| ISSN: | 0008-8749 |
| Popis: | Long-lived plasma cells (PCs) contribute to humoral immunity through an undefined mechanism. Memory B cells, but not human naïve B cells, can be induced to differentiate into long-lived PCs in vitro. Because evidence links a proliferation-inducing ligand (APRIL), a tumor necrosis factor family member, to the ability of bone marrow to mediate long-term PC survival, we reasoned that APRIL influences the proliferation and differentiation of naïve B cells. We describe here the development of a simple cell culture system that allowed us to show that APRIL sustained the proliferation of naïve human B cells and induced them to differentiate into long-lived PCs. Blocking the transmembrane activator and calcium modulator and cyclophilin ligand interactor or B-cell mature antigen shows they were required for the differentiation of naïve B cells into long-lived PCs in vitro. Our in vitro culture system will reveal new insights into the biology of long-lived PCs. |
| Databáze: | OpenAIRE |
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