New Fluorescent Macrolide Derivatives for Studying Interactions of Antibiotics and Their Analogs with the Ribosomal Exit Tunnel
| Autor: | Natalia V. Sumbatyan, Andrey G Tereshchenkov, Vyacheslav A. Chertkov, Alexey A. Bogdanov, A. V. Shishkina, V. V. Karpenko, Andrey L. Konevega, Pavel Kasatsky |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chemistry Stereochemistry 030106 microbiology General Medicine Tylosin Biochemistry Fluorescence Ribosome Rhodamine 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Escherichia coli Fluorescein BODIPY Ribosomes Fluorescence anisotropy Alexa Fluor Fluorescent Dyes |
| Zdroj: | Biochemistry. Biokhimiia. 81(10) |
| ISSN: | 1608-3040 |
| Popis: | Novel fluorescent derivatives of macrolide antibiotics related to tylosin bearing rhodamine, fluorescein, Alexa Fluor 488, BODIPY FL, and nitrobenzoxadiazole (NBD) residues were synthesized. The formation of complexes of these compounds with 70S E. coli ribosomes was studied by measuring the fluorescence polarization depending on the ribosome amount at constant concentration of the fluorescent substance. With the synthesized fluorescent tylosin derivatives, the dissociation constants for ribosome complexes with several known antibiotics and macrolide analogs previously obtained were determined. It was found that the fluorescent tylosin derivatives containing BODIPY FL and NBD groups could be used to screen the binding of novel antibiotics to bacterial ribosomes in the macrolide-binding site. |
| Databáze: | OpenAIRE |
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