Voronoi-based spatial analysis reveals selective interneuron changes in the cortex of FALS mice
| Autor: | Diego Minciacchi, Claudia Del Tongo, Roman M. Kassa, Raffaella Mariotti, Marina Bentivoglio |
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| Rok vydání: | 2009 |
| Předmět: |
Interneuron
Cell aggregation Mice Transgenic Biology Mice GABA Developmental Neuroscience Interneurons Cortex (anatomy) Neuroplasticity medicine Animals Humans Motoneuron disease Amyotrophic lateral sclerosis Motor cortex Somatosensory cortex Parvalbumin Spatial analysis Cortical plasticity Cerebral Cortex Analysis of Variance Behavior Animal Superoxide Dismutase Age Factors Disease Models Animal Parvalbumins Visual cortex medicine.anatomical_structure nervous system Neurology Cerebral cortex biology.protein Neuroscience |
| Zdroj: | Experimental Neurology. 215:77-86 |
| ISSN: | 0014-4886 |
| DOI: | 10.1016/j.expneurol.2008.09.005 |
| Popis: | The neurodegenerative disease amyotrophic lateral sclerosis affects lower motoneurons and corticospinal cells. Mice expressing human mutant superoxide dismutase (SOD)1 provide widely investigated models of the familial form of disease, but information on cortical changes in these mice is still limited. We here analyzed the spatial organization of interneurons characterized by parvalbumin immunoreactivity in the motor, somatosensory, and visual cortical areas of SOD1(G93A) mice. Cell number and sociological spatial behavior were assessed by digital charts of cell location in cortical samples, cell counts, and generation of two-dimensional Voronoi diagrams. In end-stage SOD1-mutant mice, an increase of parvalbumin-containing cortical interneurons was found in the motor and somatosensory areas (about 35% and 20%, respectively) with respect to wild-type littermates. Changes in cell spatial distribution, as documented by Voronoi-derived coefficients of variation, indicated increased tendency of parvalbumin cells to aggregate into clusters in the same areas of the SOD1-mutant cortex. Counts and coefficients of variation of parvalbumin cells in the visual cortex gave instead similar results in SOD1-mutant and wild-type mice. Analyses of motor and somatosensory areas in presymptomatic SOD1-mutant mice provided findings very similar to those obtained at end-stage, indicating early changes of interneurons in these cortical areas during the pathology. Altogether the data reveal in the SOD1-mutant mouse cortex an altered architectonic pattern of interneurons, which selectively affects areas involved in motor control. The findings, which can be interpreted as pathogenic factors or early disease-related adaptations, point to changes in the cortical regulation and modulation of the motor circuit during motoneuron disease. |
| Databáze: | OpenAIRE |
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