Molecular study of human herpesvirus 6 and 8 involvement in coronary atherosclerosis and coronary instability
| Autor: | Marco Magnoni, Domenico Russo, Mauro S. Malnati, Domenico Cianflone, Giacomo Ruotolo, Ottavio Alfieri, Nicole Cristell, Paolo Lusso, Stefano Coli, Attilio Maseri |
|---|---|
| Přispěvatelé: | Magnoni, M, Malnati, M, Cristell, N, Coli, S, Russo, D, Ruotolo, G, Cianflone, Domenico, Alfieri, Ottavio, Lusso, P, Maseri, A. |
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Acute coronary syndrome Endothelium Herpesvirus 6 Human viruses Myocardial Infarction Coronary Artery Disease Disease Real-Time Polymerase Chain Reaction Sensitivity and Specificity Pathogenesis Coronary artery disease Virology Internal medicine Prevalence Humans Medicine Viremia Myocardial infarction Coronary atherosclerosis Aged biology business.industry virus diseases Herpesviridae Infections Middle Aged Viral Load medicine.disease biology.organism_classification Coronary Vessels Cross-Sectional Studies Infectious Diseases medicine.anatomical_structure DNA Viral Herpesvirus 8 Human Cardiology Female Human herpesvirus 6 business |
| Zdroj: | Journal of Medical Virology. 84:1961-1966 |
| ISSN: | 0146-6615 |
| Popis: | "Several lines of evidence suggest the involvement of infectious agents in the pathogenesis of atherosclerosis. Furthermore, a correlation between infection-driven inflammatory burden and acute manifestation of coronary artery disease has been hypothesized. The aim of this work was to assess whether human herpesvirus (HHV)-6 and HHV-8, two DNA viruses with a distinct tropism for endothelium and lymphocytes, may be associated with coronary instability. An age- and gender-matched cross-sectional study was undertaken in 70 patients with testing of plasma HHV-6 and HHV-8 DNA load in different cardiovascular clinical settings: 29 patients with acute myocardial infarction, 21 patients with stable coronary artery disease, and 20 patients without coronary and carotid artery atherosclerosis subjected to cardiac valve replacement. In all patients, HHV-6 and HHV-8 plasma DNA was tested by using highly sensitive, calibrated quantitative real-time PCR assays which employ a synthetic DNA calibrator to adjust for DNA extraction and amplification efficiency. HHV-8 viremia was undetectable in all three groups. HHV-6 viremia was detected in a substantial fraction of the samples examined (18.6%) without significant differences among the three groups (ST segment elevation myocardial infarction: 17.2%; stable coronary artery disease: 14.3%; patients without coronary and carotid artery atherosclerosis: 25%). Furthermore, no significant differences in plasma HHV-6 load were observed amongst the three groups of patients. These findings indicate that coronary instability is not associated specifically with active HHV-6 or HHV-8 infection. However, an unusually high rate of active HHV-6 infection was documented among patients without atherosclerosis admitted to hospital with cardiac disease. " |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text