Physiological characterization of thermotolerant yeast for cellulosic ethanol production
| Autor: | Carlos J. A. de Souza, Daniela A. Costa, Hugo Leonardo André Genier, Patrícia dos Santos Costa, Ancély F. dos Santos, Luciano Gomes Fietto, Marina Quádrio Raposo Branco Rodrigues, Wendel Batista da Silveira, Mariana Lopes |
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| Jazyk: | angličtina |
| Předmět: |
Sugarcane bagasse
Saccharomyces cerevisiae Applied Microbiology and Biotechnology Industrial Microbiology Kluyveromyces Kluyveromyces marxianus Bioenergy and Biofuels Ethanol fuel Food science Cellulose biology Ethanol Chemistry Drug Tolerance General Medicine Industrial microbiology biology.organism_classification Yeast Thermotolerant yeast Biochemistry Cellulosic ethanol Fermentation Bagasse Simultaneous saccharification and fermentation Brazil Biotechnology |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV Applied Microbiology and Biotechnology |
| ISSN: | 0175-7598 |
| DOI: | 10.1007/s00253-014-5580-3 |
| Popis: | The conversion of lignocellulose into fermentable sugars is considered a promising alternative for increasing ethanol production. Higher fermentation yield has been achieved through the process of simultaneous saccharification and fermentation (SSF). In this study, a comparison was performed between the yeast species Saccharomyces cerevisiae and Kluyveromyces marxianus for their potential use in SSF process. Three strains of S. cerevisiae were evaluated: two are widely used in the Brazilian ethanol industry (CAT-1 and PE-2), and one has been isolated based on its capacity to grow and ferment at 42 °C (LBM-1). In addition, we used thermotolerant strains of K. marxianus. Two strains were obtained from biological collections, ATCC 8554 and CCT 4086, and one strain was isolated based on its fermentative capacity (UFV-3). SSF experiments revealed that S. cerevisiae industrial strains (CAT-1 and PE-2) have the potential to produce cellulosic ethanol once ethanol had presented yields similar to yields from thermotolerant strains. The industrial strains are more tolerant to ethanol and had already been adapted to industrial conditions. Moreover, the study shows that although the K. marxianus strains have fermentative capacities similar to strains of S. cerevisiae, they have low tolerance to ethanol. This characteristic is an important target for enhancing the performance of this yeast in ethanol production. |
| Databáze: | OpenAIRE |
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