Genetic diversity of the Metarhizium anisopliae complex in Colima, Mexico, using microsatellites
| Autor: | Adrien Gallou, María G. Serna-Domínguez, Patricia Castro-Félix, Hugo César Arredondo-Bernal, Rogelio Rosas-Valdez, Gilda Y. Andrade-Michel |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Metarhizium
Species complex Insecta Genotype Metarhizium anisopliae Polymerase Chain Reaction 03 medical and health sciences Peptide Elongation Factor 1 Intergenic region Sensu Genetics Animals DNA Fungal Mexico Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Genetic diversity biology 030306 microbiology Genetic Variation Sequence Analysis DNA Plants biology.organism_classification Infectious Diseases Microsatellite Metarhizium acridum Sequence Alignment Microsatellite Repeats |
| Zdroj: | Fungal Biology. 123:855-863 |
| ISSN: | 1878-6146 |
| DOI: | 10.1016/j.funbio.2019.09.005 |
| Popis: | Metarhizium anisopliae is a complex of cryptic species with wide geographical distribution and versatile lifestyles. In this study, 45 isolates of the Metarhizium genus harbored in the “Coleccion de Hongos Entomopatogenos” of the “Centro Nacional de Referencia de Control Biologico” from different substrates, insect-host, and localities from Colima, Mexico, were phylogenetically identified using the 5′end of translation elongation factor 1-α (5′TEF) and intergenic nuclear region MzFG543igs. Seven species were recognized, M. acridum (n = 26), M. pemphigi (n = 1), and within the PARB and MGT clades: M. anisopliae (N = 7; sensu stricto: n = 2; sensu lato: n = 5), M. brunneum (n = 2), M. guizhouense (n = 2), M. pingshaense (n = 2), and M. robertsii (n = 5). Twenty-nine SSR markers were developed for M. acridum; according to the analysis of 12 polymorphic SSR loci, M. acridum showed low genetic diversity, revealing five genotypes with a dominant one (n = 21). Based on the analysis of 13 specific SSR loci, 14 genotypes were identified within the PARB and MGT clades. This study contributes to generating valuable information about the community structure and genotypic diversity of Metharhizum species in the state of Colima, Mexico. |
| Databáze: | OpenAIRE |
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