IL-6 augments angiotensinogen in primary cultured renal proximal tubular cells
| Autor: | Ryousuke Satou, L. Gabriel Navar, Hiroyuki Kobori, Kayoko Miyata, Romer A. Gonzalez-Villalobos, Naro Ohashi, Omar W. Acres, Maki Urushihara |
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| Rok vydání: | 2009 |
| Předmět: |
STAT3 Transcription Factor
medicine.medical_specialty Time Factors Interleukin-1beta Angiotensinogen Biochemistry Article Antibodies Kidney Tubules Proximal Endocrinology Internal medicine parasitic diseases medicine Humans RNA Messenger Interleukin 6 STAT3 Receptor Molecular Biology Cells Cultured Regulation of gene expression Messenger RNA biology Interleukin-6 Tumor Necrosis Factor-alpha Angiotensin II NF-kappa B Epithelial Cells NFKB1 Receptors Interleukin-6 Gene Expression Regulation biology.protein Tumor necrosis factor alpha |
| Zdroj: | Molecular and Cellular Endocrinology. 311:24-31 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2009.06.013 |
| Popis: | In human kidneys, the mechanisms underlying angiotensinogen (AGT) augmentation by interleukin 6 (IL-6) are poorly understood and the only information available is in HK-2, immortalized human renal proximal tubular epithelial cells. Therefore, the present study was performed to elucidate the effects of IL-6 on AGT expression in primary cultured human renal proximal tubular epithelial cells (RPTEC) after characterization of HK-2 and RPTEC. RPTEC showed low basal AGT mRNA (11+/-1%) and protein (7.0+/-0.9%) expression, high IL-6 receptor (IL-6R) expression (282+/-17%), and low basal NF-kappaB (43+/-7%) and STAT3 (43+/-7%) activities compared to those in HK-2. In RPTEC, AGT mRNA and protein expressions were enhanced by IL-6 (172+/-31% and 378+/-39%, respectively). This AGT augmentation was attenuated by an IL-6R antibody. STAT3 phosphorylation (366+/-55% at 30min) and translocation were enhanced by IL-6. The AGT augmentation was attenuated by a STAT3 inhibitor. These data indicate that IL-6 increases AGT expression via STAT3 pathway in RPTEC. |
| Databáze: | OpenAIRE |
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