Epitope mapping of Aleutian mink disease parvovirus virion protein VP1 and 2
| Autor: | J Christensen, A Holm, N Steenfos, F Costello, E Gottschalck, K T Jensen, Bent Aasted |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
viruses
Immunology Molecular Sequence Data Aleutian Mink Disease Antibodies Viral Capsid biology.animal Animals Amino Acid Sequence Mink Peptide sequence Gene Expression vector Linear epitope biology Immunodominant Epitopes Virion virus diseases Aleutian Mink Disease Virus General Medicine Fusion protein Virology Molecular biology Recombinant Proteins Epitope mapping biology.protein Capsid Proteins Female Antibody Epitope Mapping |
| Zdroj: | Scandinavian journal of immunology. 49(4) |
| ISSN: | 0300-9475 |
| Popis: | Six overlapping fragments of the Aleutian Mink Disease parvoVirus (AMDV) virion protein VP1 and 2 (VP1/2) gene were inserted into the expression vector pMAL-c2. Four of the clones carried large overlapping fragments covering the entire VP1/2 gene. The remaining two clones covered specifically chosen regions within the VP1/2 gene. Using a Western blotting detection system, sera from AMDV-infected mink were tested against the recombinant polypeptides. These studies showed reactions primarily directed against the two AMDV polypeptides ranging from amino acids 297 to 518. Weaker reactions against other regions of the VP1/2 were also observed. The small fusion protein designed to cover the presumed AMDV VP1/2 loop 4 was purified by affinity chromatography and used to develop solid-phase immunoassays. Twelve small synthetic peptides were constructed and used as inhibitors. A peptide covering amino acids S428 to T448 was shown to block the reactivity of a pool of positive mink sera, indicating the presence of one dominant linear epitope. |
| Databáze: | OpenAIRE |
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