Monitoring structural features, biocompatibility and biological efficacy of gamma-irradiated methotrexate-loaded spray-dried microparticles
| Autor: | Philippe C. Mesquita, Alice R. Oliveira, Yêda Medeiros Bastos de Almeida, Matheus F. Fernandes-Pedrosa, Eryvaldo Sócrates Tabosa do Egito, Alianda Maira Cornélio, Paula Renata Lima Machado, Kleber Juvenal Silva Farias, Arnóbio Antônio da Silva-Júnior |
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| Rok vydání: | 2017 |
| Předmět: |
Drug
Materials science Biocompatibility Chemistry Pharmaceutical media_common.quotation_subject Bioengineering 02 engineering and technology Pharmacology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Drug Delivery Systems medicine Humans Lactic Acid Irradiation Particle Size media_common Spray dried Biological activity 021001 nanoscience & nanotechnology Microspheres 0104 chemical sciences PLGA Methotrexate chemistry Gamma Rays Mechanics of Materials Particle size 0210 nano-technology Polyglycolic Acid medicine.drug Biomedical engineering |
| Zdroj: | Materials Science and Engineering: C. 80:438-448 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2017.06.013 |
| Popis: | In this study, biodegradable and biocompatible gamma irradiated poly-(dl-lactide-co-glycolide) (PLGA) spray-dried microparticles were prepared aiming to improve the efficacy of methotrexate (MTX). The experimental design included three formulations of microparticles containing distinct drug amount (9%, 18%, and 27% w/w) and three distinct gamma irradiation dose (15kGy, 25kGy, and 30kGy). The physicochemical and drug release properties of the microparticles supported their biocompatibility and biological efficacy studies in different cell lines. The irradiation induced slight changes in the spherical shape of the microparticles and the formation of free radicals was dependent on the drug loading. However, the amorphous character, particle size, drug loading, and drug release rate of the microparticles were preserved. The drug release data from all microparticles formulation were evaluated by using four drug kinetic models and by comparison of their similarity factor (f2). The gamma irradiation did not induce changes in the biocompatibility of PLGA microparticles and in the biological activity of the MTX-loaded microparticles. Finally, the spray-dried MTX-loaded PLGA microparticles enhanced the efficacy of the drug in the human cervical cancer cells (SiHa cell line). This study demonstrated the feasibility of the gamma irradiated spray dried PLGA microparticles for prolonged release of MTX, supporting a promising antitumor-drug delivery system for parenteral (subcutaneous) or pulmonary use. |
| Databáze: | OpenAIRE |
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