Corticosterone and testosterone treatment influence expression of gene pathways linked to meiotic segregation in preovulatory follicles of the domestic hen

Autor: Alexandra B. Bentz, Kristen J. Navara, Stephen T Gardner, W. Walter Lorenz, Elizabeth R. Wrobel, Mary T. Mendonça
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Physiology
Gene Expression
01 natural sciences
Biochemistry
Chromosome segregation
Polar body
chemistry.chemical_compound
Endocrinology
Ovarian Follicle
Corticosterone
Reproductive Physiology
Medicine and Health Sciences
Testosterone
Lipid Hormones
Multidisciplinary
Sex Chromosomes
Meiotic spindle organization
Chromosome Biology
Bird Genetics
Z Chromosomes
Eukaryota
Meiosis
Vertebrates
Androgens
Medicine
Female
Research Article
Ovulation
Offspring
Science
Biology
010603 evolutionary biology
Chromosomes
Andrology
Birds
03 medical and health sciences
Population Metrics
Germinal disc
Genetics
Animals
Sex Ratio
Gene
Menstrual Cycle
Population Biology
Endocrine Physiology
Organisms
Biology and Life Sciences
Cell Biology
Hormones
030104 developmental biology
chemistry
Gene Expression Regulation
Amniotes
Animal Genetics
Chickens
Zdroj: PLoS ONE, Vol 15, Iss 5, p e0232120 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Decades of work indicate that female birds can control their offspring sex ratios in response to environmental and social cues. In laying hens, hormones administered immediately prior to sex chromosome segregation can exert sex ratio skews, indicating that these hormones may act directly on the germinal disc to influence which sex chromosome is retained in the oocyte and which is discarded into an unfertilizable polar body. We aimed to uncover the gene pathways involved in this process by testing whether treatments with testosterone or corticosterone that were previously shown to influence sex ratios elicit changes in the expression of genes and/or gene pathways involved in the process of meiotic segregation. We injected laying hens with testosterone, corticosterone, or control oil 5h prior to ovulation and collected germinal discs from the F1 preovulatory follicle in each hen 1.5h after injection. We used RNA-sequencing (RNA-seq) followed by DESeq2 and gene set enrichment analyses to identify genes and gene pathways that were differentially expressed between germinal discs of control and hormone-treated hens. Corticosterone treatment triggered downregulation of 13 individual genes, as well as enrichment of gene sets related to meiotic spindle organization and chromosome segregation, and additional gene sets that function in ion transport. Testosterone treatment triggered upregulation of one gene, and enrichment of one gene set that functions in nuclear chromosome segregation. This work indicates that corticosterone can be a potent regulator of meiotic processes and provides potential gene targets on which corticosterone and/or testosterone may act to influence offspring sex ratios in birds.
Databáze: OpenAIRE
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