Effects of anesthesia on renal function and metabolism in rats assessed by hyperpolarized MRI
| Autor: | Christian Østergaard Mariager, Christoffer Laustsen, Haiyun Qi, Jakob Lindhardt, Hans Stødkilde-Jørgensen, Per Mose Nielsen |
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| Rok vydání: | 2017 |
| Předmět: |
Renal function
Hemodynamics Kidney Fluanisone Sevoflurane 030218 nuclear medicine & medical imaging Fentanyl Anesthesia Procedure 03 medical and health sciences 0302 clinical medicine medicine Image Processing Computer-Assisted Animals Radiology Nuclear Medicine and imaging Anesthesia Rats Wistar Thiopental business.industry Butyrophenones Magnetic Resonance Imaging Rats Glucose Renal blood flow Anesthetics Inhalation Midazolam Female business 030217 neurology & neurosurgery Anesthetics Intravenous medicine.drug |
| Zdroj: | Qi, H, Mariager, C Ø, Lindhardt, J, Nielsen, P M, Stødkilde-Jørgensen, H & Laustsen, C 2018, ' Effects of anesthesia on renal function and metabolism in rats assessed by hyperpolarized MRI ', Magnetic Resonance in Medicine . https://doi.org/10.1002/mrm.27165 |
| ISSN: | 1522-2594 |
| Popis: | PURPOSE: Anesthesia is necessary for most animal studies requiring invasive procedures. It is well documented that various types of anesthesia modulate a wide variety of important metabolic and functional processes in the body, and as such, represent a potential limitation in the study design. In the present study, we aimed to investigate the renal functional and metabolic consequences of 3 typical rodent anesthetics used in preclinical MRI: sevoflurane, inaction, and a mixture of fentanyl, fluanisone, and midazolam (FFM).METHODS: The renal effects of 3 different classes of anesthetics (inactin, servoflurane, and FFM) were investigated using functional and metabolic MRI. The renal glucose metabolism and hemodynamics was characterized with hyperpolarized [1-13C]pyruvate MRI and by DCE imaging.RESULTS: Rats receiving sevoflurane or FFM had blood glucose levels that were 1.3-fold to 1.4-fold higher than rats receiving inactin. A 2.9-fold and 4.8-fold increased13C-lactate/13C-pyruvate ratio was found in the FFM mixture anesthetized group compared with the sevoflurane and the inactin anesthetized groups. The FFM anesthesia resulted in a 50% lower renal plasma flow compared with the sevoflurane and the inactin anesthetized groups.CONCLUSION: This study demonstrates different renal metabolic and hemodynamic changes under 3 different anesthetics, using hyperpolarized MR in rats. Inactin and sevoflurane were found to affect the renal hemodynamic and metabolic status to a lesser degree than FFM. Sevoflurane anesthesia is particularly easy to induce and maintain during the whole anesthesia procedure, and as such, represents a good alternative to inaction, although it alters the blood glucose level. |
| Databáze: | OpenAIRE |
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