In vitro and in vivo cytotoxicity assessment of glyphosate and imazethapyr-based herbicides and their association

Autor: Gessyca Costa, Andréia Fernandes, Thaís Santos, Lara Brito, Laís Rodrigues, Marize Valadares, Israel Felzenszwalb, Elisa Ferraz, Daniela Morais Leme, Gisele Oliveira
Přispěvatelé: Universidade Federal de Goiás (UFG), State University of Rio de Janeiro, Fluminense Federal University, Universidade Estadual Paulista (UNESP), Federal University of Paraná
Rok vydání: 2022
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1087-2620
1528-7394
Popis: Made available in DSpace on 2022-04-28T19:51:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Resistance to glyphosate herbicide has initiated usage of combined application of herbicides as a weed control measure. Imazethapyr-based herbicides associated with glyphosate herbicide seem to be an alternative to suppress weed resistance. The aim of this study was to examine the adverse effects of Glyphosate Atanor 48® (ATN) and Imazethapyr Plus Nortox® (IMZT) formulations in both single forms and mixtures using HepG2 cells and zebrafish early-life stages models. Data demonstrated cytotoxicity due to exposure to ATN, IMZT for both models, as follows: (1) ATN (0.5 mg/L), IMZT (5 mg/L), and M3 (0.05 mg/L ATN + 5 mg/L IMZT) increased cytotoxicity by disturbing the mitochondrial activity of HepG2 cells 24 hr after exposure; (2) ATN and IMZT (5 mg/L), and M3 (0.05 mg/L ATN + 5 mg/L IMZT) also decreased the integrity of the membrane of HepG2 cells after 24 hr incubation; (3) only ATN and IMZT (5 mg/L) in their single forms diminished the mitochondrial potential of zebrafish; (4) ATN (single form) at 0.5 mg/L induced apoptosis in zebrafish larvae. In conclusion, these herbicides in their single forms appeared to produce greater cytotoxicity to HepG2 cells and zebrafish compared to the herbicide mixtures. Environmental Toxicology Research Laboratory Faculty of Pharmacy Federal University of Goiás Department of Biophysics and Biometry Institute of Biology Roberto Alcantara Gomes State University of Rio de Janeiro Laboratory of Teaching and Research in Toxicology in Vitro Faculty of Pharmacy Federal University of Goiás Department of Pharmacy and Pharmaceutical Administration Pharmacy College Fluminense Federal University Institute of Chemistry National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives UNESP Departament of Genetics Federal University of Paraná Institute of Chemistry National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives UNESP
Databáze: OpenAIRE
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