Replacement by a lacZ reporter gene assigns mouse connexin36, 45 and 43 to distinct cell types in pancreatic islets
| Autor: | Klaus Willecke, Olaf Krüger, Achim Plum, Pedro Luis Herrera, Britta Döring, Dorothée Caille, Anne Charollais, Martin Theis, Virginie Nepote, Christopher Brink, Joachim Degen, Christophe Mas, Paolo Meda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Gene isoform
Recombinant Fusion Proteins/analysis Recombinant Fusion Proteins Transgene Endothelium Vascular/chemistry Cre recombinase Mice Transgenic Biology Connexins/ analysis/genetics/metabolism Connexins Islets of Langerhans Mice Genes Reporter Insulin Secretion medicine Animals Insulin Coding region Gene ddc:616 Insulin/secretion Reporter gene Reverse Transcriptase Polymerase Chain Reaction Pancreatic islets Gene targeting Cell Biology beta-Galactosidase Molecular biology medicine.anatomical_structure Connexin 43 Gene Targeting Connexin 43/ analysis/genetics cardiovascular system Endothelium Vascular sense organs Islets of Langerhans/blood supply/ chemistry/cytology biological phenomena cell phenomena and immunity Beta-Galactosidase/analysis/ genetics |
| Zdroj: | Experimental Cell Research Experimental Cell Research, Vol. 294, No 1 (2004) pp. 18-29 |
| ISSN: | 0014-4827 |
| Popis: | Transcripts of three connexin isoforms (Cx36, Cx43 and Cx45) have been reported in rodent pancreatic islets, but the precise distribution of the cognate proteins is still unknown. We determined expression of Cx36 in a cell-autonomous manner using mice with a targeted replacement of the Cx36 coding region by a lacZ reporter gene. For cell-autonomous monitoring of Cx43 expression, we used the Cre/loxP system: Mice carrying the Cx43 coding region flanked by loxP sites (floxed) also carried an embedded lacZ gene that is activated after Cre-mediated recombination in cells with transcriptional activity of the Cx43 gene. Deletion of the Cx43 coding region in beta-cells did not result in the activation of the embedded lacZ reporter gene. Instead, Cx43 expression was found in endothelial cells of the islets of Langerhans in mice with endothelium-specific deletion. Ubiquitous deletion of Cx43 led to a similar endothelial lacZ expression, but again, activity of the reporter gene was not detected in beta-cells. Mice with targeted replacement of the Cx45 coding region by lacZ showed a vascular expression similar to Cx43. The data show that native insulin-producing cells express a connexin isoform (Cx36) which differs from those (Cx43 and Cx45) expressed by vascular islet cells. |
| Databáze: | OpenAIRE |
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