Type IV pili, transient bacterial aggregates, and virulence of enteropathogenic Escherichia coli
| Autor: | Rosemary Fernandez, Sandra W. Ramer, Toru Tobe, Cheng-Yen Wu, William J. Murray, David Bieber, Gary K. Schoolnik |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Diarrhea Agglutination Adolescent Fimbria Virulence Pilus retraction Biology medicine.disease_cause Pilus Bacterial Adhesion Bacterial genetics Microbiology Operon medicine Escherichia coli Tumor Cells Cultured Humans Enteropathogenic Escherichia coli Intestinal Mucosa Escherichia coli Infections Multidisciplinary Cell Membrane Epithelial Cells Middle Aged biology.organism_classification Enterobacteriaceae Phenotype Fimbriae Bacterial Mutation |
| Zdroj: | Science (New York, N.Y.). 280(5372) |
| ISSN: | 0036-8075 |
| Popis: | Type IV bundle-forming pili of enteropathogenic Escherichia coli are required for the localized adherence and autoaggregation phenotypes. Whether these pili are also required for virulence was tested in volunteers by inactivating bfpA or bfpT ( perA ) encoding, respectively, the pilus subunit and the bfp operon transcriptional activator. Both mutants caused significantly less diarrhea. Mutation of the bfpF nucleotide-binding domain caused increased piliation, enhanced localized adherence, and abolished the twitching motility–dispersal phase of the autoaggregation phenotype. The bfpF mutant colonized the human intestine but was about 200-fold less virulent. Thus, BfpF is required for dispersal from the bacterial aggregate and for full virulence. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|