Association between polymorphisms in the biometabolism genes CYP1A1, GSTM1, GSTT1 and GSTP1 in bladder cancer
| Autor: | Roberta Losi-Guembarovski, Marco Aurelio de Freitas Rodrigues, Farid Libos Júnior, Paulo Emilio Fuganti, Ilce Mara de Syllos Cólus, Iara S. Rodrigues, Rodrigo Paes de Menezes, Henrique Mitsu Matsuda, Hellen Kuasne, João Paulo Souto Grando, Emerson Pereira Gregório |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Oncology medicine.medical_specialty Biology urologic and male genital diseases Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology GSTP1 Internal medicine Molecular genetics Genotype Cytochrome P-450 CYP1A1 medicine Humans Genetic Predisposition to Disease Allele Carcinogen Aged Glutathione Transferase Aged 80 and over Genetics Bladder cancer General Medicine Odds ratio Middle Aged medicine.disease Glutathione S-Transferase pi Urinary Bladder Neoplasms Case-Control Studies Female Variants of PCR Polymorphism Restriction Fragment Length |
| Zdroj: | Clinical and Experimental Medicine. 9:21-28 |
| ISSN: | 1591-9528 1591-8890 |
| DOI: | 10.1007/s10238-008-0015-z |
| Popis: | Numerous enzymes, including Cytochrome P450s (phase I) and Glutathione-S-transferases (phase II), are involved in the metabolic activation and detoxification of carcinogens. Epidemiological studies have consistently demonstrated that bladder cancer is strongly associated with cigarette smoking, and the risk for the development of this neoplasia may be modified by individual differences in carcinogen-metabolizing genes. We investigated the relationship between polymorphisms in the CYP1A1, GSTM1, GSTT1, and GSTP1 genes in a case-control study with 100 bladder cancer patients and 100 controls matched for age, gender, race, and smoking status. The GSTM1, GSTT1, CYP1A1 (A2455--G), and GSTP1 (A313--G) genotypes were determined using a multiplex PCR, an allele specific PCR, and a restriction fragment length polymorphism-PCR method. The present case-controlled association study did not detect any positive or negative association for the GSTM1 and GSTP1 genes [odds ratios (OR) = 1.35; 95% confidence interval (CI) = 0.76-2.41 and OR = 0.75; 95% CI = 0.41-1.38, respectively]. Notably, the genes GSTT1 and CYP1A1 exhibited a statistically significant association with bladder cancer (OR = 1.77; 95% CI = 1.01-3.12 and OR = 1.99; 95% CI = 1.07-3.73). No differences for GSTM1 and GSTP1 genotype prevalence between the bladder cancer cases and the controls were observed, however, the null genotype for the GSTT1 gene and the A/G and G/G variants of the CYP1A1 gene may contribute to the development of bladder cancer. |
| Databáze: | OpenAIRE |
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