Direct and transdentinal biostimulatory effects of grape seed extract rich in proanthocyanidin on pulp cells
| Autor: | P. A. O. Silva, J. M. Pacheco, Patricia N R Pereira, A. F. dos Santos, Taia Maria Berto Rezende, Ana K. Bedran-Russo, A. Ribeiro |
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| Rok vydání: | 2018 |
| Předmět: |
food.ingredient
Cellular differentiation 0206 medical engineering 02 engineering and technology Cell morphology Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food Humans Proanthocyanidins Food science General Dentistry Dental Pulp Grape Seed Extract Chemistry Cell Differentiation 030206 dentistry 020601 biomedical engineering Proanthocyanidin Cell culture Grape seed extract Dentin Alkaline phosphatase Pulp (tooth) |
| Zdroj: | International Endodontic Journal. 52:424-438 |
| ISSN: | 0143-2885 |
| Popis: | AIM To evaluate the potential biostimulatory effects of grape seed extract (GSE) on a primary culture of human pulp cells. METHODOLOGY Human molars were used to obtain the primary pulp cell culture and 0.5-mm dentine discs. For GSE direct exposure, dose-response (0.0065-6.5%) and time response (1-60 min of contact) were examined. For transdentinal exposure, 0.65% of GSE was tested for 24 h. Cellular metabolism, nitric oxide and collagen production, and cell morphology alterations were assessed at periods of 24 and 72 h. After cell differentiation and direct exposure to GSE, the total protein production (TP), alkaline phosphatase activity (ALP) and formation of mineralization nodules (MN) were assessed. The results were analysed by parametric tests or non-parametric tests (α = 0.05). RESULTS The lower concentration of GSE tested (0.0065%) was associated with an increase in cellular metabolism, a reduction in the production of nitric oxide and an increase in extracellular matrix synthesis (collagen). Distinct behaviours were observed for the different concentrations, without a reduction of cellular metabolism >10% compared with the control, either when applied directly or transdentinally. SEM revealed no significant change in cell morphology, except for the positive control (H2 O2 ). There was no difference in TP, ALP or MN between the control group and the group exposed to GSE. CONCLUSIONS Treatment with grape seed extract, even at the highest concentration and longest period, caused neither direct nor transdentinal cytotoxic effects on human pulp cells. Grape seed extract components may play a biostimulatory role and protect dental pulp cells when in direct contact. |
| Databáze: | OpenAIRE |
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