The role of senescence-associated gene101 (PagSAG101a) in the regulation of secondary xylem formation in poplar
Autor: | Dian Wang, Mengzhu Lu, Cheng Jiang, Guo-Hua Chai, Hui He, Shuang-Shuang Wen, Xueqin Song, Ying-Li Liu, Shutang Zhao |
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Rok vydání: | 2021 |
Předmět: |
Zinc finger
Cambium Cell division fungi food and beverages Xylem Plant Science Biology biology.organism_classification Plants Genetically Modified Biochemistry Wood General Biochemistry Genetics and Molecular Biology Cell biology Transcriptome Populus Cell Wall Gene Expression Regulation Plant Arabidopsis thaliana Secondary cell wall Woody plant Plant Proteins |
Zdroj: | Journal of integrative plant biologyREFERENCES. 64(1) |
ISSN: | 1744-7909 |
Popis: | Wood is produced by accumulation of secondary xylem via proliferation and differentiation of the cambium cells in woody plants. Identifying the regulators involved in this process remains a challenging task. In this study, we isolated PagSAG101a, the homolog of Arabidopsis thaliana SAG101, from a hybrid poplar (Populus alba × P. glandulosa) clone 84K and investigated its role in secondary xylem development. PagSAG101a was expressed predominantly in lignified stems and localized in the nucleus. Compared with non-transgenic 84K plants, transgenic plants overexpressing PagSAG101a displayed increased plant height, internode number, stem diameter, xylem width, and secondary cell wall thickness, while opposite phenotypes were observed for PagSAG101a knock-out plants. Transcriptome analyses revealed that differentially expressed genes were enriched for those controlling cambium cell division activity and subsequent secondary cell wall deposition during xylem formation. In addition, the tandem CCCH zinc finger protein PagC3H17, which positively regulates secondary xylem width and secondary wall thickening in poplar, could bind to the promoter of PagSAG101a and mediate the regulation of xylem differentiation. Our results support that PagC3H17, downstream of PagC3H17, functions in wood development. This article is protected by copyright. All rights reserved. |
Databáze: | OpenAIRE |
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