Bothrops Jararaca snake venom modulates key Cancer-related proteins in breast tumor cell lines
| Autor: | Leo Kei Iwai, Daniela Cajado-Carvalho, Stephanie Santos Suehiro Arcos, Hamida Macêdo Calacina, Emer S. Ferro, Milton Yutaka Nishiyama-Jr, Ismael Feitosa Lima, Wellington da Silva Santos, Fabio Montoni, Carolina Yukiko Kisaki |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Proteomics Bothrops jararaca Cell Survival Health Toxicology and Mutagenesis proteome Venom Breast Neoplasms Biology Pharmacology Toxicology complex mixtures Article 03 medical and health sciences 0302 clinical medicine breast cancer Cell Line Tumor Crotalid Venoms medicine Humans Protein Interaction Maps mass spectrometry snake venom Cell growth Cancer biology.organism_classification medicine.disease Neoplasm Proteins Gene Expression Regulation Neoplastic 030104 developmental biology Gene Ontology Snake venom 030220 oncology & carcinogenesis Cancer cell Proteome CÉLULAS MORTAS Medicine |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Toxins Volume 13 Issue 8 Toxins, Vol 13, Iss 519, p 519 (2021) |
| Popis: | Cancer is characterized by the development of abnormal cells that divide in an uncontrolled way and may spread into other tissues where they may infiltrate and destroy normal body tissue. Several previous reports have described biochemical anti-tumorigenic properties of crude snake venom or its components, including their capability of inhibiting cell proliferation and promoting cell death. However, to the best of our knowledge, there is no work describing cancer cell proteomic changes following treatment with snake venoms. In this work we describe the quantitative changes in proteomics of MCF7 and MDA-MB-231 breast tumor cell lines following treatment with Bothrops jararaca snake venom, as well as the functional implications of the proteomic changes. Cell lines were treated with sub-toxic doses at either 0.63 μg/mL (low) or 2.5 μg/mL (high) of B. jararaca venom for 24 h, conditions that cause no cell death per se. Proteomics analysis was conducted on a nano-scale liquid chromatography coupled on-line with mass spectrometry (nLC-MS/MS). More than 1000 proteins were identified and evaluated from each cell line treated with either the low or high dose of the snake venom. Protein profiling upon venom treatment showed differential expression of several proteins related to cancer cell metabolism, immune response, and inflammation. Among the identified proteins we highlight histone H3, SNX3, HEL-S-156an, MTCH2, RPS, MCC2, IGF2BP1, and GSTM3. These data suggest that sub-toxic doses of B. jararaca venom have potential to modulate cancer-development related protein targets in cancer cells. This work illustrates a novel biochemical strategy to identify therapeutic targets against cancer cell growth and survival. |
| Databáze: | OpenAIRE |
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