Immunohistochemical detection of insulin-like growth factor-I, transforming growth factor-beta2, basic fibroblast growth factor and epidermal growth factor-receptor expression in developing rat ovary
| Autor: | Kemal Ergin, E. Gürsoy, Yücel Başımoğlu Koca, Hatice Kübra Başaloğlu, Kamil Seyrek |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Immunology Basic fibroblast growth factor Ovary Biochemistry chemistry.chemical_compound Insulin-like growth factor Transforming Growth Factor beta2 Internal medicine Follicular phase medicine Immunology and Allergy Animals Epidermal growth factor receptor Insulin-Like Growth Factor I Rats Wistar Molecular Biology biology Growth factor Hematology Immunohistochemistry Rats ErbB Receptors medicine.anatomical_structure Endocrinology chemistry biology.protein Female Fibroblast Growth Factor 2 Immunostaining Transforming growth factor |
| Zdroj: | Cytokine. 43(2) |
| ISSN: | 1096-0023 |
| Popis: | The aim of this study was to determine the immunohistochemical expression and localization of insulin-like growth factor-I (IGF-I), transforming growth factor-β2 (TGF-β2), basic fibroblast growth factor (bFGF) and epidermal growth factor-receptor (EGF-R) in developing rat ovaries. Eighteen female Wistar rats were enrolled in this study; newborn ( n = 6), one-month-old ( n = 6) and adult ( n = 6) rats. Formalin-fixed and parafin-embedded ovarian tissues were stained with antibodies against IGF-I, TGF-β2, bFGF and EGF-R, immunohistochemically. The ovarian cells were evaluated by semi-quantitative scoring system under light microscope. The staining of IGF-I, TGF-β2, bFGF and EGF-R were most intense in the oocytes and were heavily at one-month-old rats. A moderate immunostaining in theca cells and corpus luteii reacted with IGF-I in adult rats. Furthermore the staining intensity for IGF-I was moderate in granulosa cells of newborn rat ovaries. We detected also a moderate staining for TGF-β2 in corpus luteii of adult rats. In addition, we found a bFGF immunostaining mainly in oocytes of follicles of young and adult rats. Immunostaining for EGF-R was moderate in granulosa cells of one-month-old rats. In conclusion, this study suggests that growth factors play a pivotal role in ovarian function, especially in follicular development. The role of growth factor in controlling degeneration or growth (or both) of ovary follicles remain as explained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect