Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis
| Autor: | Valérie Lefebvre, Jacques Trouverie, Marie-Noelle Fortabat, Mylène Durand-Tardif, Aloïse Ducamp, Emilie Gineau, Catherine Lapierre, Grégory Mouille, Alexia Guillebaux, Stéphane Herbette, Christine Horlow, Matthieu Bensussan, Delphine Naquin, Aurélie Baldy |
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| Přispěvatelé: | Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Centre de génétique moléculaire UPR 3404, Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Institut Jean-Pierre Bourgin ( IJPB ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier ( PIAF ), Institut National de la Recherche Agronomique ( INRA ) -Université Blaise Pascal - Clermont-Ferrand 2 ( UBP ), Ecophysiologie Végétale, Agronomie et Nutritions ( EVA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Physiology
[SDV]Life Sciences [q-bio] Mutant Molecular Sequence Data Arabidopsis Dwarfism Plant Science Phloem Suppression Genetic Acetyltransferases Cell Wall Xylem Genetics Vascular cambium medicine Arabidopsis thaliana [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Biomass Cellulose Glucuronidase biology [ SDV ] Life Sciences [q-bio] Arabidopsis Proteins fungi Wild type Membrane Proteins Water food and beverages Acetylation Articles biology.organism_classification medicine.disease Cell biology Phenotype Ethyl Methanesulfonate Mutation Plant Vascular Bundle |
| Zdroj: | Plant Physiology Plant Physiology, 2015, 168 (2), pp.452-463. ⟨10.1104/pp.15.00122⟩ Plant Physiology, American Society of Plant Biologists, 2015, 168 (2), pp.452-463. ⟨10.1104/pp.15.00122⟩ Plant Physiology, American Society of Plant Biologists, 2015, 168 (2), pp.452-463. 〈10.1104/pp.15.00122〉 |
| ISSN: | 0032-0889 1532-2548 |
| Popis: | eskimo1-5 (esk1-5) is a dwarf Arabidopsis (Arabidopsis thaliana) mutant that has a constitutive drought syndrome and collapsed xylem vessels, along with low acetylation levels in xylan and mannan. ESK1 has xylan O-acetyltransferase activity in vitro. We used a suppressor strategy on esk1-5 to screen for variants with wild-type growth and low acetylation levels, a favorable combination for ethanol production. We found a recessive mutation in the KAKTUS (KAK) gene that suppressed dwarfism and the collapsed xylem character, the cause of decreased hydraulic conductivity in the esk1-5 mutant. Backcrosses between esk1-5 and two independent knockout kak mutants confirmed suppression of the esk1-5 effect. kak single mutants showed larger stem diameters than the wild type. The KAK promoter fused with a reporter gene showed activity in the vascular cambium, phloem, and primary xylem in the stem and hypocotyl. However, suppression of the collapsed xylem phenotype in esk1 kak double mutants was not associated with the recovery of cell wall O-acetylation or any major cell wall modifications. Therefore, our results indicate that, in addition to its described activity as a repressor of endoreduplication, KAK may play a role in vascular development. Furthermore, orthologous esk1 kak double mutants may hold promise for ethanol production in crop plants. |
| Databáze: | OpenAIRE |
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