Galleria mellonella infection model to evaluate pathogenic and nonpathogenic Leptospira strains
| Autor: | Xiaojuan Zhu, Sreekumari Rajeev, Dhani Prakoso |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Serotype
Leptospira animal structures General Veterinary Strain (chemistry) Virulence Host (biology) fungi General Medicine Biology Moths biology.organism_classification Microbiology Pathogenesis Galleria mellonella Rodent Diseases Disease Models Animal Cricetinae Larva Animals Leptospirosis Leptospira interrogans serovar Copenhageni |
| Zdroj: | Veterinary microbiology. 264 |
| ISSN: | 1873-2542 |
| Popis: | The Galleria mellonella larvae infection model is emerging as a valuable tool for studying various characteristics of infectious agents and host-pathogen interaction. This system has been widely recognized as a high throughput, ethical, and cost-effective invertebrate infection model to study the virulence and pathogenesis of various bacterial pathogens. In this study, we compared the effect of Leptospira infection in G. mellonella larvae infected with Leptospira interrogans serovar Copenhageni (pathogenic) or Leptospira biflexa serovar Patoc (saprophytic) strains. We observed significant pathologic changes such as decreased activity, complete melanization, and lower survival rate in the G. mellonella larvae infected with a pathogenic strain L. interrogans serovar Copenhageni compared to those infected with a nonpathogenic strain L. biflexa serovar Patoc. Our study demonstrates the feasibility and the potential of using G. mellonella larvae as an alternative model to study virulence mechanisms and pathogenesis of Leptospira strains. Once optimized, the G. mellonella infection model can be a potential substitute for hamsters to explore various host and pathogen-related mechanistic events in Leptospira infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect