Inhibition of RNA and Protein Synthesis by Ethanol in Regenerating Rat Liver: Evidence for Transcriptional Inhibition of Protein Synthesis
| Autor: | A. R. Pösö, H. Pösö |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Transcription Genetic Aldehyde dehydrogenase Biology Toxicology Ornithine decarboxylase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tyrosine aminotransferase Protein biosynthesis Animals Hepatectomy Nucleotide 030304 developmental biology Alcohol dehydrogenase Fomepizole Orotic Acid Pharmacology chemistry.chemical_classification 0303 health sciences Ethanol RNA Rats Inbred Strains DNA-Directed RNA Polymerases Molecular biology Liver Regeneration Rats Liver Biochemistry chemistry Protein Biosynthesis 030220 oncology & carcinogenesis biology.protein Pyrazoles |
| Zdroj: | Acta Pharmacologica et Toxicologica. 49:125-129 |
| ISSN: | 0001-6683 |
| DOI: | 10.1111/j.1600-0773.1981.tb00880.x |
| Popis: | The effect of ethanol on RNA and protein synthesis was studied in regenerating rat liver. Partial hepatectomy stimulated the activity of ornithine decarboxylase (ODC) and that of tyrosine aminotransferase (TAT) as well as the synthesis of RNA. The intubation of ethanol (3 g/kg) 1 hr before the operation inhibited the stimulation of ODC and TAT when measured 4 hrs after the operation. Ethanol administration also inhibited RNA synthesis. By using inhibitors of alcohol dehydrogenase and aldehyde dehydrogenase it was shown that the inhibition of RNA synthesis (and also that of protein synthesis) was most probably a direct effect of ethanol itself. When RNA synthesis was measured in vitro in purified nuclei, ethanol inhibited the incorporation of nucleotides to RNA. It is suggested that ethanol inhibits protein synthesis in regenerating rat liver at the transcriptional level by interfering in nuclei with the synthesis of RNA. |
| Databáze: | OpenAIRE |
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