Parameters optimization for enzymatic assays using experimental design
| Autor: | Janaína Fernandes de Medeiros Burkert, F. Maugeri Filho, Susana Juliano Kalil, Maria Isabel Rodrigues |
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| Rok vydání: | 2006 |
| Předmět: |
Inulinase activity
factorial design and response surface analysis Chromatography Sucrose biology Chemistry activity General Chemical Engineering Inulin lcsh:TP155-156 Substrate (chemistry) Factorial experiment inulinase biology.organism_classification Activity Enzymatic Assays chemistry.chemical_compound Kluyveromyces marxianus Response surface analysis Factorial design lcsh:Chemical engineering Inulinase |
| Zdroj: | Repositório Institucional da FURG (RI FURG) Universidade Federal do Rio Grande (FURG) instacron:FURG Brazilian Journal of Chemical Engineering, Vol 23, Iss 2, Pp 163-170 (2006) Brazilian Journal of Chemical Engineering v.23 n.2 2006 Brazilian Journal of Chemical Engineering Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ Brazilian Journal of Chemical Engineering, Volume: 23, Issue: 2, Pages: 163-170, Published: JUN 2006 |
| ISSN: | 0104-6632 |
| Popis: | The conditions for maximization enzymatic activity were determined using experimental design and inulinase from Kluyveromyces marxianus ATCC 16045. The effects of substrate concentration (sucrose and inulin), pH and temperature on inulinase activity were verified using four factorial design and surface response analysis. Using sucrose as substrate. It has bean shown that the effects sucrose on enzymatic activity is not statistically significant and the best condition for the highest activity (110 U/mL) was achieved with temperature between 60°C and 68°C and pH between 4.5 and 5.0. Using inulin as substrate it was verified that temperature is the only variable statistically significant and the maximum activity was 7.3 U/mL at temperature between 50°C and 51°C. |
| Databáze: | OpenAIRE |
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