N-Acyl Homoserine Lactones and Lux Solos Regulate Social Behaviour and Virulence of Pseudomonas syringae pv. actinidiae
| Autor: | Joel L. Vanneste, Irene Donati, Francesco Spinelli, Annalisa Polverari, Antonio Cellini, Luca Fiorentini, Elodie Vandelle, Giampaolo Buriani, Vittorio Venturi |
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| Přispěvatelé: | Cellini A., Donati I., Fiorentini L., Vandelle E., Polverari A., Venturi V., Buriani G., Vanneste J.L., Spinelli F. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology Homoserine Pseudomonas syringae Soil Science Virulence Acyl-Butyrolactones AHL Actinidia chinensis Bacterial canker Kiwifruit Quorum sensing Microbiology 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Actinidia chinensi Pathogen Ecology Evolution Behavior and Systematics Plant Diseases Acyl-Homoserine Lactones Ecology biology food and beverages biochemical phenomena metabolism and nutrition biology.organism_classification Repressor Proteins 030104 developmental biology chemistry Trans-Activators Microbial Interactions Proteobacteria Phyllosphere Transcription Factors |
| Popis: | The phyllosphere is a complex environment where microbes communicate through signalling molecules in a system, generally known as quorum sensing (QS). One of the most common QS systems in Gram-negative proteobacteria is based on the production of N-acyl homoserine lactones (AHLs) by a LuxI synthase and their perception by a LuxR sensor. Pseudomonas syringae pv. actinidiae (Psa), the aetiological agent of the bacterial canker of kiwifruit, colonises plant phyllosphere before penetrating via wounds and natural openings. Since Psa genome encodes three LuxR solos without a cognate LuxI, this bacterium may perceive diffusible signals, but it cannot produce AHLs, displaying a non-canonical QS system. The elucidation of the mechanisms underlying the perception of environmental cues in the phyllosphere by this pathogen and their influence on the onset of pathogenesis are of crucial importance for a long-lasting and sustainable management of the bacterial canker of kiwifruit. Here, we report the ability of Psa to sense its own population density and the presence of surrounding bacteria. Moreover, we show that Psa can perceive AHLs, indicating that AHL-producing neighbouring bacteria may regulate Psa virulence in the host. Our results suggest that the ecological environment is important in determining Psa fitness and pathogenic potential. This opens new perspectives in the use of more advanced biochemical and microbiological tools for the control of bacterial canker of kiwifruit. |
| Databáze: | OpenAIRE |
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