The direct and indirect association of cervical microbiota with the risk of cervical intraepithelial neoplasia

Autor: Yunong Gao, Weijiao Gao, Ying Liu, Jing Shen, Hongchao Xiong, Chaoting Zhang, Yaqi Pan
Rok vydání: 2018
Předmět:
0301 basic medicine
HPV
China
Cancer Research
16sRNA
cervical microbiota
next‐generation sequencing
Uterine Cervical Neoplasms
Atopobium vaginae
Cervix Uteri
cervical intraepithelial neoplasia
urologic and male genital diseases
medicine.disease_cause
Cervical intraepithelial neoplasia
03 medical and health sciences
0302 clinical medicine
RNA
Ribosomal
16S
Lactobacillus
medicine
Humans
Radiology
Nuclear Medicine and imaging
Normal cytology
Papillomaviridae
neoplasms
Original Research
Bacteria
Base Sequence
biology
business.industry
Microbiota
Papillomavirus Infections
HPV infection
virus diseases
Sequence Analysis
DNA
Uterine Cervical Dysplasia
medicine.disease
biology.organism_classification
female genital diseases and pregnancy complications
Highly sensitive
surgical procedures
operative
030104 developmental biology
Oncology
Streptococcus agalactiae
030220 oncology & carcinogenesis
Immunology
Female
Bacteroides fragilis
business
Cancer Prevention
Zdroj: Cancer Medicine
ISSN: 2045-7634
DOI: 10.1002/cam4.1471
Popis: Cervical microbiota composition is associated with cervical HPV infection and CIN severity. Previous studies only assessed the total association between cervical microbiota and HPV infections or CINs, and yet no study reported the direct and indirect associations between cervical microbiota and CINs mediated by HPV infection, respectively. The aim of this study was to investigate the direct and indirect associations between microbiotas and CIN severity. Cervical microbiota of 126 women with CIN 1− (normal cytology and CIN 1) and 40 with CIN 2+ (CIN 2 and CIN 3) were analyzed using Illumina sequencing based on the 16S rRNA gene. HPV was detected using a highly sensitive PCR primer set (SPF1/GP6+). Indirect effects of Pseudomonas stutzeri, Bacteroides fragilis, Lactobacillus delbrueckii, Atopobium vaginae, and Streptococcus agalactiae mediated by HPV infection on CIN status were observed. The directions of the direct and the indirect associations between CIN status and Ps. stutzeri were opposite. The directions of the direct and the indirect associations between CIN status and A. vaginae were the same. B. fragilis, L. delbrueckii, and S. agalactiae only had indirect association with CIN status. In summary, our study provided suggestive evidence that some microbial populations could have direct or indirect effects mediated by affecting HPV infection on CIN progression. Besides HPV infection, microbial community composition possibly plays a role in cervical carcinogenesis.
Databáze: OpenAIRE
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