Expression analysis of melatonin receptor subtypes in the ovary of domestic chicken
| Autor: | J. S. Tyagi, M. Saxena, M. Sudhagar, N. R. Sundaresan, D. Anish, Marie Dennis Leo, Vishesh Kumar Saxena, Jaganathan Subramani, K.V.H. Sastry, Khawaja Ashfaque Ahmed |
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| Rok vydání: | 2008 |
| Předmět: |
endocrine system
medicine.medical_specialty Receptors Melatonin Ovary Biology Melatonin receptor Melatonin Downregulation and upregulation hemic and lymphatic diseases Internal medicine Follicular phase medicine Animals RNA Messenger Cloning Molecular Receptor neoplasms General Veterinary Gene Expression Profiling General Medicine carbohydrates (lipids) Endocrinology medicine.anatomical_structure Gene Expression Regulation Theca Female Chickens hormones hormone substitutes and hormone antagonists medicine.drug Hormone |
| Zdroj: | Veterinary research communications. 33(1) |
| ISSN: | 0165-7380 |
| Popis: | Melatonin (N-acetyl-5-methoxytryptamine), an indole hormone, regulates various biological functions through three different receptor subtypes (Mel-1a, Mel-1b, and Mel-1c). However, the distribution of different melatonin receptor subtypes in chicken reproductive tissues was not known. In the present investigation, the partial sequences of ovarian melatonin receptor subtypes (Mel-1a, Mel-1b, and Mel-1c) were characterized. Further, the expression profile of melatonin receptor subtypes in the granulosa and theca layers of different preovulatory and postovulatory follicles (POF) were studied by semi-quantitative RT-PCR. The expression of all three subtypes of melatonin receptors were observed in the ovary of domestic chicken. Analysis of partial sequences of ovarian melatonin receptors revealed that the melatonin subtypes were identical to the brain receptors. In small white ovary follicles, we observed only the expression of mel-1b receptors, but not mel-1a or mel-1c receptors. In yellow follicles, all the three subtypes of receptors expression were noticed. Interestingly, we observed the expression of mel-1a receptor only in thecal layer, but not in granulosa layer. In contrast, mel-1b and -1c receptors were expressed in both granulosa and thecal layer. During the regression of POF, we observed significant upregulation of melatonin receptors (mel-1a and 1c) expression, that downregulated in the later stages of regression. We assume that the expression of melatonin receptors might have been influenced by the atresia or apoptosis of different follicular layers in POF. Our findings suggest that the differential distribution of melatonin receptor subtypes might have distinct downstream cellular functions in the ovarian tissues. |
| Databáze: | OpenAIRE |
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