Okadaic acid co-induces vimentin expression and cell cycle arrest in MPC-11 mouse plasmacytoma cells
| Autor: | Annemarie Scherbarth, Peter Traub, Günter Giese, Manfred Kubbies, Wolfram Wiegers |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Cell cycle checkpoint
Physiology Clinical Biochemistry Phosphatase Fluorescent Antibody Technique Vimentin chemistry.chemical_compound Mice Ethers Cyclic Okadaic Acid Tumor Cells Cultured Animals Protein Phosphatase Inhibitor Microscopy Confocal biology Cell Cycle Protein phosphatase 1 Cell Biology Okadaic acid Cell cycle Molecular biology Cell biology chemistry Microscopy Fluorescence Tetradecanoylphorbol Acetate biology.protein Carcinogens Plasmacytoma |
| Zdroj: | Journal of Cellular Physiology |
| Popis: | The effect of the tumor promoter okadaic acid on cell cycle progression and on vimentin expression in MPC-11 mouse plasmacytoma cells was compared with that of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Cell cycle progression of asynchronously grown MPC-11 cells was inhibited by both agents, but, in contrast to the G1 phase arrest caused by TPA, okadaic acid gave rise to G2/M phase and S phase arrest. This effect of okadaic acid was delayed significantly compared to the TPA-caused arrest. Furthermore, okadaic acid was able to induce vimentin expression to an extent comparable to the TPA response. However, vimentin expression was markedly delayed in okadaic acid-treated relative to TPA-treated cells. Another protein phosphatase inhibitor, calyculin A, also induced cell cycle changes and vimentin expression at concentrations at or above 1 x 10(-9) M. Based on these observations, we suggest an involvement of protein phosphatase 1 (possibly also phosphatase 2A and/or other phosphatases) in both the G2/M cell cycle block and the induction of vimentin expression in MPC-11 cells by okadaic acid. |
| Databáze: | OpenAIRE |
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