Transcription in vitro from a DNA template containing apurinic sites
| Autor: | Margaret D. Mamet-Bratley |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Time Factors
Transcription Genetic Alkylation Biology Nucleic Acid Denaturation Coliphages Biochemistry Genetics and Molecular Biology (miscellaneous) chemistry.chemical_compound Transcription (biology) Escherichia coli A-DNA AP site Carbon Radioisotopes Nucleic Acid Hybridization DNA-Directed RNA Polymerases Templates Genetic Hydrogen-Ion Concentration Molecular biology Adenosine Monophosphate In vitro Kinetics chemistry Biochemistry Purines DNA Viral RNA Viral Depurination Electrophoresis Polyacrylamide Gel DNA |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 340:237-243 |
| ISSN: | 0005-2787 |
| DOI: | 10.1016/0005-2787(74)90269-x |
| Popis: | T7 DNA containing a limited number of apurinic sites shows reduced template activity for RNA synthesis in vitro. Initiation specificity remains unchanged by the presence of apurinic sites. Quantitative analysis shows that depurination is much more detrimental than alkylation to the template activity of DNA. Such a result is in accord with phage-lethality studies which show, for monofunctional alkylating agents, that depurination is much more toxic than alkylation. |
| Databáze: | OpenAIRE |
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