Characterization of a new human monoclonal antibody directed against the Vel antigen
| Autor: | Sophie Danard, Y. Danger, F. Vérité, Vanessa Gringoire, P. Riou, G. Semana, T. Peyrard |
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| Přispěvatelé: | Etablissement français du sang [Rennes] (EFS Bretagne), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie Intégrée du Globule Rouge (BIGR (UMR_S_1134 / U1134)), Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Université des Antilles (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Microenvironnement et cancer (MiCa), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Jonchère, Laurent |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.drug_class [SDV]Life Sciences [q-bio] 030204 cardiovascular system & hematology Biology Monoclonal antibody Vel blood group antigen Flow cytometry Serology 03 medical and health sciences 0302 clinical medicine Western blot Antigen medicine Humans medicine.diagnostic_test Antibodies Monoclonal Membrane Proteins SMIM1 Hematology General Medicine Molecular biology 3. Good health [SDV] Life Sciences [q-bio] 030104 developmental biology Monoclonal Blood Group Antigens biology.protein Female human monoclonal antibody Antibody Clone (B-cell biology) |
| Zdroj: | Vox Sanguinis Vox Sanguinis, Wiley, 2016, 110 (2), pp.172-178. ⟨10.1111/vox.12321⟩ Vox Sanguinis, 2016, 110 (2), pp.172-178. ⟨10.1111/vox.12321⟩ |
| ISSN: | 0042-9007 1423-0410 |
| DOI: | 10.1111/vox.12321 |
| Popis: | International audience; BACKGROUND AND OBJECTIVES: The Vel blood group antigen is a poorly characterized high-prevalence antigen. Until now, anti-Vel antibodies have been observed in only alloimmunized Vel-negative individuals. In this study, we aimed to establish a human hybridoma cell line secreting the first anti-Vel monoclonal antibody (mAb), clone SpG213Dc. MATERIALS AND METHODS: Peripheral blood lymphocytes from a French Vel-negative woman with anti-Vel in her plasma were transformed with Epstein-Barr virus and then hybridized with the myeloma cell line Sp2/O-Ag14 using the polyethylene glycol (PEG) method. A specific anti-Vel mAb was successfully produced and was extensively characterized by serological, flow cytometry and Western blot analyses. RESULTS: One human anti-Vel-secreting clone was produced and the secreted anti-Vel mAb (SpG213Dc) was examined. The specificity of the SpG213Dc mAb was assessed by its reactivity against a panel of nine genotyped RBCs including, respectively, three Vel-negative and six Vel-positive (three wild-type homozygous and three heterozygous) samples using flow cytometry method. Vel-positive RBCs were specifically stained and were subsequently used to perform Western blot and immunoprecipitation analysis of the Vel antigen. CONCLUSION: Serological characterization of the new monoclonal anti-Vel SpG213Dc showed a heterogeneous level of expression of the Vel antigen on the different RBCs. Our results suggest that the mAb SpG213Dc can be reliably used as a blood grouping reagent, thus allowing the mass-scale phenotyping of blood donors to strengthen rare blood banks with Vel-negative RBC units |
| Databáze: | OpenAIRE |
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