Anticonvulsant agents, dizocilpine maleate, enadoline and HA 966 have different effects on N-methyl-DL-aspartate-induced immediate early gene induction in mice
| Autor: | John Hughes, J.A. Poat, Geoffrey N. Woodruff, Ryszard J. Oles, V.L. Woodburn |
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| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Silver Staining N-Methylaspartate Pyrrolidines Gene Expression Mice Inbred Strains In situ hybridization Biology Receptors N-Methyl-D-Aspartate chemistry.chemical_compound Mice Internal medicine Gene expression medicine Animals RNA Messenger Genes Immediate-Early In Situ Hybridization Benzofurans Brain Chemistry Messenger RNA General Neuroscience Enadoline Brain Pyrrolidinones Dizocilpine Endocrinology chemistry NMDA receptor Anticonvulsants HA-966 Dizocilpine Maleate Immediate early gene medicine.drug |
| Zdroj: | Neuroscience. 56(3) |
| ISSN: | 0306-4522 |
| Popis: | Administration of N-methyl- dl -aspartate (85 mg/ml) was given by infusion (0.14ml/min) until a clonic seizure was elicited. In situ hybridization was used to assess regional levels of four immediate early gene messenger RNA levels (c- fos , c- jun , jun B, and a nerve growth factor induced gene, NGFI-A). Messenger RNA levels were highest at 25 min following infusion of N-methyl- dl -aspartate . c- jun messenger RNA levels remained elevated for over 2 h; however, c- fos , jun B and, NGFI-A messenger RNA levels had returned to control levels by this time. Expression was detected in the hippocampus, hypothalamus and piriform cortex. Pre-treatment (30 min prior to N-methyl- dl -aspartate ) with the anticonvulsant drugs dizocilpine maleate (1 mg/kg) and HA 966 (200 μg, i.c.v) resulted in significantly reduced immediate early gene messenger RNA levels in the hypothalamus and piriform cortex, and attenuated levels in the hippocampus. Pre-treatment with the anticonvulsant agent enadoline (3 mg/kg), given at an anticonvulsant dose, did not result in reduced immediate early gene messenger RNA levels. These results suggest that monitoring immediate early gene expression may lead to advances in the understanding of the mechanism of action of many pharmacological agents, such as the kappa-opioid agonist enadoline. |
| Databáze: | OpenAIRE |
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