Methods for Studying Site-Specific O-GlcNAc Modifications: Successes, Limitations, and Important Future Goals

Autor:	Stuart P. Moon, Afraah Javed, Eldon R. Hard, Matthew R. Pratt
Rok vydání:	2021
Předmět:	Chemistry protein synthesis chemical tools Perspective O-GlcNAc QD1-999 Post-translational modifications protein modification
Zdroj:	JACS Au JACS Au, Vol 2, Iss 1, Pp 74-83 (2021)
ISSN:	2691-3704
Popis:	O-GlcNAcylation is a dynamic post-translational modification which affects myriad proteins, cellular functions, and disease states. Its presence or absence modulates protein function via differential protein- and site-specific mechanisms, necessitating innovative techniques to probe the modification in highly selective manners. To this end, a variety of biological and chemical methods have been developed to study specific O-GlcNAc modification events both in vitro and in vivo, each with their own respective strengths and shortcomings. Together, they comprise a potent chemical biology toolbox for the analysis of O-GlcNAcylation (and, in theory, other post-translational modifications) while highlighting the need and space for more facile, generalizable, and biologically authentic techniques.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c10c5751766bd56d4b0473cf20c8377b https://doi.org/10.1021/jacsau.1c00455 Zobrazit plný text záznamu