Sse8387I, a new type-II restriction endonuclease that recognizes the octanucleotide sequence 5′-CCTGCAGG-3′
| Autor: | Yukiko Kawashima, Hiroaki Ito, Makoto Takagi, Yoshiko Nomura, Hiroaki Sagawa, Akihiko Kita, Ikunosin Kato, Hirokazu Kotani |
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| Rok vydání: | 1990 |
| Předmět: |
DNA
Bacterial viruses Molecular Sequence Data Simian virus 40 Genome Restriction fragment chemistry.chemical_compound Genetics Animals Bacteriophages Nucleotide Deoxyribonucleases Type II Site-Specific chemistry.chemical_classification Base Sequence biology Adenoviruses Human Lambda phage biology.organism_classification Bacteriophage lambda Molecular biology Streptomyces PBR322 Restriction enzyme Restriction site Oligodeoxyribonucleotides chemistry DNA Viral biology.protein DNA Plasmids |
| Zdroj: | Nucleic Acids Research. 18:5637-5640 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/18.19.5637 |
| Popis: | A type II restriction endonuclease designated Sse8387I was partially purified from Streptomyces sp. 8387. This enzyme cleaved adenovirus 2 DNA at three sites, lambda phage DNA at five sites, and pUC18 and M13mp18 RF DNA at one site each, but did not cleave the DNAs from pBR322, SV40, or phi X174. Sse8387I recognized the octanucleotide sequence 5'-CCTGCA decreases GG-3', cleaving where shown by the arrow. Sse8387I is the first restriction endonuclease to be reported that recognizes an octanucleotide sequence consisting of all four nucleotides, G, A, T, and C. The frequency of occurrence of Sse8387I sites within sequenced regions of primate genomes was 2.4 times that of NotI sites. |
| Databáze: | OpenAIRE |
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