Targeting of ICAM-1 on vascular endothelium under static and shear stress conditions using a liposomal Gd-based MRI contrast agent
| Autor: | Lucas W. E. Starmans, Nynke M. S. van den Akker, Daniel G. M. Molin, Leonie E. M. Paulis, Gustav J. Strijkers, Igor Jacobs, Klaas Nicolay, Tessa Geelen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Pathology
Contrast Media Medicine (miscellaneous) Pharmaceutical Science Gadolinium 02 engineering and technology SDG 3 – Goede gezondheid en welzijn Applied Microbiology and Biotechnology Mice Drug Delivery Systems Immune Regulation [NCMLS 2] Leukocytes 0303 health sciences ICAM-1 Liposome medicine.diagnostic_test Intercellular Adhesion Molecule-1 021001 nanoscience & nanotechnology Magnetic Resonance Imaging Molecular Imaging medicine.anatomical_structure lcsh:R855-855.5 Molecular Medicine medicine.symptom Shear Strength 0210 nano-technology medicine.medical_specialty lcsh:Medical technology Endothelium MRI contrast agent lcsh:Biotechnology Biomedical Engineering Inflammation Bioengineering Molecular MRI Cell Line 03 medical and health sciences SDG 3 - Good Health and Well-being In vivo lcsh:TP248.13-248.65 medicine Animals 030304 developmental biology Shear stress Tumor Necrosis Factor-alpha business.industry Research Endothelial Cells Magnetic resonance imaging Leukocyte Immunoglobulin G Liposomes Biophysics Stress Mechanical business |
| Zdroj: | Journal of Nanobiotechnology, 10, 25-25 Journal of Nanobiotechnology, Vol 10, Iss 1, p 25 (2012) Journal of Nanobiotechnology, 10, 1, pp. 25-25 Journal of Nanobiotechnology, 10, 1-12. BioMed Central Journal of Nanobiotechnology |
| ISSN: | 1477-3155 |
| Popis: | Background The upregulation of intercellular adhesion molecule-1 (ICAM-1) on the endothelium of blood vessels in response to pro-inflammatory stimuli is of major importance for the regulation of local inflammation in cardiovascular diseases such as atherosclerosis, myocardial infarction and stroke. In vivo molecular imaging of ICAM-1 will improve diagnosis and follow-up of patients by non-invasive monitoring of the progression of inflammation. Results A paramagnetic liposomal contrast agent functionalized with anti-ICAM-1 antibodies for multimodal magnetic resonance imaging (MRI) and fluorescence imaging of endothelial ICAM-1 expression is presented. The ICAM-1-targeted liposomes were extensively characterized in terms of size, morphology, relaxivity and the ability for binding to ICAM-1-expressing endothelial cells in vitro. ICAM-1-targeted liposomes exhibited strong binding to endothelial cells that depended on both the ICAM-1 expression level and the concentration of liposomes. The liposomes had a high longitudinal and transversal relaxivity, which enabled differentiation between basal and upregulated levels of ICAM-1 expression by MRI. The liposome affinity for ICAM-1 was preserved in the competing presence of leukocytes and under physiological flow conditions. Conclusion This liposomal contrast agent displays great potential for in vivo MRI of inflammation-related ICAM-1 expression. |
| Databáze: | OpenAIRE |
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