A duplex pcr assay for the detection of ralstonia solanacearum phylotype ii strains in musa spp
| Autor: | Philippe Prior, Aude Chabirand, Gilles Cellier, Aurélie Moreau, Bruno Hostachy, Florent Ailloud |
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| Přispěvatelé: | French Agcy Food Environm & Occupat Hlth & Safety, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), UAR 0865 Paris Réunion, Institut National de la Recherche Agronomique (INRA)-Direction des Ressources Humaines (DRH)-Paris Réunion (PARIS REUNION), French Agency for Food, Environmental and Occupational Health & Safety (ANSES) - Plant Health Laboratory (LSV) - Tropical Pests and Diseases unit |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Phylogénie Identification [SDV]Life Sciences [q-bio] lcsh:Medicine Musa (bananes) Polymerase Chain Reaction 01 natural sciences law.invention Ralstonia law Lignée Musa (plantains) lcsh:Science Pathogen Phylogeny Polymerase chain reaction 2. Zero hunger Phylotype 0303 health sciences Ralstonia solanacearum Multidisciplinary biology Phylogenetic tree food and beverages Biodiversity PCR banana blood-disease bacterium Brazil Research Article top nov Sensitivity and Specificity Diagnostic de laboratoire Microbiology 03 medical and health sciences Phylogenetics pseudomonas-syzygii molecular plant pathology Plant Diseases H20 - Maladies des plantes 030306 microbiology wilt proposal lcsh:R Reproducibility of Results Musa biology.organism_classification Musaceae lcsh:Q U30 - Méthodes de recherche 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2015, 10 (3), pp.1-17. ⟨10.1371/journal.pone.0122182⟩ PloS One Plos One 3 (10), 1-17. (2015) PLoS ONE, Vol 10, Iss 3, p e0122182 (2015) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0122182⟩ |
| Popis: | International audience; Banana wilt outbreaks that are attributable to Moko disease-causing strains of the pathogen Ralstonia solanacearum (Rs) remain a social and economic burden for both multinational corporations and subsistence farmers. All known Moko strains belong to the phylotype II lineage, which has been previously recognized for its broad genetic basis. Moko strains are paraphyletic and are distributed among seven related but distinct phylogenetic clusters (sequevars) that are potentially major threats to Musaceae, Solanaceae, and ornamental crops in many countries. Although clustered within the Moko IIB-4 sequevar, strains of the epidemiologically variant IIB-4NPB do not cause wilt on Cavendish or plantain bananas; instead, they establish a latent infection in the vascular tissues of plantains and demonstrate an expanded host range and high aggressiveness toward Solanaceae and Cucurbitaceae. Although most molecular diagnostic methods focus on strains that wilt Solanaceae (particularly potato), no relevant protocol has been described that universally detects strains of the Musaceae-infecting Rs phylotype II. Thus, a duplex PCR assay targeting Moko and IIB-4NPB variant strains was developed, and its performance was assessed using an extensive collection of 111 strains representing the known diversity of Rs Moko-related strains and IIB-4NPB variant strains along with certain related strains and families. The proposed diagnostic protocol demonstrated both high accuracy (inclusivity and exclusivity) and high repeatability, detected targets on either pure culture or spiked plant extracts. Although they did not belong to the Moko clusters described at the time of the study, recently discovered banana-infecting strains from Brazil were also detected. According to our comprehensive evaluation, this duplex PCR assay appears suitable for both research and diagnostic laboratories and provides reliable detection of phylotype II Rs strains that infect Musaceae. |
| Databáze: | OpenAIRE |
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