Dual excitation wavelength system for combined fingerprint and high wavenumber Raman spectroscopy
| Autor: | Christine M. O'Brien, Jennifer L. Herington, Anita Mahadevan-Jansen, Isaac J. Pence, Ton G. van Leeuwen, Laura E. Masson, Jeff Reese |
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| Přispěvatelé: | Biomedical Engineering and Physics, ACS - Atherosclerosis & ischemic syndromes |
| Rok vydání: | 2018 |
| Předmět: |
Gelatin/chemistry
Materials science Analytical chemistry Spectrum Analysis Raman/instrumentation Cervix Uteri Spectrum Analysis Raman 01 natural sciences Biochemistry Phantoms Article Analytical Chemistry law.invention Imaging 010309 optics symbols.namesake Mice Fingerprint law Pregnancy 0103 physical sciences Electrochemistry Environmental Chemistry Wavenumber Animals Spectroscopy Raman/instrumentation Phantoms Imaging Spectrum Analysis 010401 analytical chemistry Detector Cervix Uteri/metabolism Water Laser 0104 chemical sciences Wavelength Molecular vibration symbols Water/analysis Gelatin Female Collagen Raman spectroscopy Excitation Collagen/chemistry |
| Zdroj: | Analyst, 143(24), 6049-6060. Royal Society of Chemistry |
| ISSN: | 1364-5528 0003-2654 |
| Popis: | A fiber optic probe-based Raman spectroscopy system using a single laser module with two excitation wavelengths, at 680 and 785 nm, has been developed for measuring the fingerprint and high wavenumber regions using a single detector. This system is simpler and less expensive than previously reported configurations of combined fingerprint and high wavenumber Raman systems, and its probe-based implementation facilitates numerous in vivo applications. The high wavenumber region of the Raman spectrum ranges from 2800–3800 cm(−1) and contains valuable information corresponding to the molecular vibrations of proteins, lipids, and water, which is complimentary to the biochemical signatures found in the fingerprint region (800–1800 cm(−1)), which probes DNA, lipids, and proteins. The efficacy of the system is demonstrated by tracking changes in water content in tissue-mimicking phantoms, where Voigtian decomposition of the high wavenumber water peak revealed a correlation between the water content and type of water-tissue interactions in the samples. This dual wavelength system was then used for in vivo assessment of cervical remodeling during mouse pregnancy, a physiologic process with known changes in tissue hydration. The system shows that Raman spectroscopy is sensitive to changes in collagen content in the fingerprint region and hydration state in the high wavenumber region, which was verified using an ex vivo comparison of wet and dry weight. Simultaneous fingerprint and high wavenumber Raman spectroscopy will allow precise in vivo quantification of tissue water content in the high wavenumber region, paired with the high biochemical specificity of the fingerprint region. |
| Databáze: | OpenAIRE |
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