Exposure to sub-10 nm particles emitted from a biodiesel-fueled diesel engine: In vitro toxicity and inflammatory potential
| Autor: | Mariano Sirignano, Paola Pedata, Vincenzo Guida, Livia Malorni, Claudia Petrarca, G. Genovese |
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| Přispěvatelé: | Malorni, L., Guida, V., Sirignano, M., Genovese, G., Petrarca, C., Pedata, P., Malorni, Livia, Guida, Vincenzo, Sirignano, Mariano, Genovese, Giuliana, Petrarca, Claudia, Pedata, Paola |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival bcl-X Protein Apoptosis 010501 environmental sciences Toxicology Diesel engine complex mixtures 01 natural sciences 03 medical and health sciences Diesel fuel Biodiesel Diesel Nanoparticles Health effects Cytokines Nanoparticle Cell Line Tumor Ultrafine particle Humans Diesel Particle Size Cytokine A549 Cell Vehicle Emissions Cell Proliferation 0105 earth and related environmental sciences Vehicle Emission Pollutant Air Pollutants Biodiesel Caspase 3 Chemistry 030111 toxicology Apoptosi General Medicine Particulates Health effect HaCaT bcl-2 Homologous Antagonist-Killer Protein A549 Cells Air Pollutant Biofuel Environmental chemistry Nanoparticles Cytokines Myeloid Cell Leukemia Sequence 1 Protein Particulate Matter Health effects Gasoline Human |
| Zdroj: | Toxicology letters 270 (2017): 51–61. doi:10.1016/j.toxlet.2017.02.009 info:cnr-pdr/source/autori:Malorni, Livia; Guida, Vincenzo; Sirignano, Mariano; Genovese, Giuliana; Petrarca, Claudia; Pedata, Paola/titolo:Exposure to sub-10 nm particles emitted from a biodiesel-fueled diesel engine: In vitro toxicity and inflammatory potential/doi:10.1016%2Fj.toxlet.2017.02.009/rivista:Toxicology letters/anno:2017/pagina_da:51/pagina_a:61/intervallo_pagine:51–61/volume:270 |
| ISSN: | 0378-4274 |
| Popis: | Objectives The inflammatory effects of organic sub-10 nm particles generated and emitted from a diesel engine fueled with a biodiesel and a commercial diesel oil are analyzed in this paper. Diesel combustion is the major sources of ultrafine particles (UFP) in the environment, particularly in urbanized areas. In the last years, there is an increasing use of biomass-derived fuels because they are a renewable source of energy that may mitigate climate change through the reduction of net CO 2 with respect to conventional fossil fuels. Although there is a general agreement on biofuels ability to reduce conventional pollutants, new and potentially harmful pollutants can be formed during biofuel combustion. In particular, the emission of sub-10 nm particles is strongly increased with respect to that of larger soot particles. Methods Organic sub-10 nm particles are separated from larger sizes particulate matter by collection in water suspension for toxicological and inflammatory tests. After exposure to sub-10 nm particles, the effects on proliferation, apoptosis and secretion of cytokines, chemokines and growth factors networks production is analyzed in immortalized non-tumorigenic human dermal keratinocyte cell line (HaCaT) and human alveolar epithelial-like cells (A549). Results and conclusion Nanoparticles exert different cytotoxic effects in the two cell lines, suggesting that the dermal way of exposure is more sensitive than the inhalant way. These differences are most evident in the secretion of pro-inflammatory, angiogenic and proliferative cytokines and chemokines whose expression is more finely modulated in HaCaT cells compared to A-549 cells. Considering the size of these particles, it is important to promote the culture of prevention also for the dermal way in particularly exposed workers. |
| Databáze: | OpenAIRE |
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