Evolving a Thermostable Terminal Deoxynucleotidyl Transferase

Autor:	Trina Faye Osothprarop, Maybelle K. Go, Wen Shan Yew, Saurabh Nirantar, Alexander G Karabadzhak, Sergio G. Peisajovich, Jasmine Puay Suan Chua, Mcdonald Seth
Rok vydání:	2020
Předmět:	0106 biological sciences endocrine system Oligonucleotides Biomedical Engineering DNA Single-Stranded DNA-Directed DNA Polymerase Protein Engineering 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Catalysis 03 medical and health sciences chemistry.chemical_compound DNA Nucleotidylexotransferase 010608 biotechnology Escherichia coli Animals Nucleotide Amino Acid Sequence 030304 developmental biology Thermostability chemistry.chemical_classification 0303 health sciences Template free Temperature General Medicine Protein engineering Kinetics stomatognathic diseases Terminal deoxynucleotidyl transferase chemistry Biochemistry Mutagenesis Feature (computer vision) Cattle Mutant Proteins DNA Plasmids
Zdroj:	ACS Synthetic Biology. 9:1725-1735
ISSN:	2161-5063
Popis:	Terminal deoxynucleotidyl transferase (TdT) catalyzes template free incorporation of arbitrary nucleotides onto single-stranded DNA. Due to this unique feature, TdT is widely used in biotechnology and clinical applications. One particularly tantalizing use is the synthesis of long
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c103693f08799c4a1c60f0cd8e4c3fcd https://doi.org/10.1021/acssynbio.0c00078 Zobrazit plný text záznamu