Purification and characterization of rhodanese from Acinetobacter calcoaceticus

Autor: Peter A. Vandenbergh, Richard S. Berk
Rok vydání: 1980
Předmět:
Zdroj: Canadian Journal of Microbiology. 26:281-286
ISSN: 1480-3275
0008-4166
DOI: 10.1139/m80-047
Popis: Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was found to be constitutively present as an intracellular enzyme in Acinetobacter calcoaceticus. The soluble enzyme was purified 40.9-fold by a procedure which included ultracentrifugation, ethanol precipitation, CM-Sephadex batchwise separation, QAE-50 ion exchange chromatography, and sucrose density gradient ultracentrifugation. The enzyme had a molecular weight of approximately 35 000 with a pH optimum of 8–8.5. Activity was substantially enhanced by supplements of 2-mercaptoethanol and to a lesser extent by cysteine–HCl or reduced glutathione. No degradation of the enzyme into smaller subunits was observed when treated with 2-mercaptoethanol.
Databáze: OpenAIRE