Purification and characterization of rhodanese from Acinetobacter calcoaceticus
Autor: | Peter A. Vandenbergh, Richard S. Berk |
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Rok vydání: | 1980 |
Předmět: |
Cyanide
Immunology Ion chromatography Rhodanese Applied Microbiology and Biotechnology Microbiology Substrate Specificity Cell-free system chemistry.chemical_compound Genetics Molecular Biology Thiosulfate Chromatography Acinetobacter Cell-Free System biology Chemistry Temperature General Medicine Hydrogen-Ion Concentration biology.organism_classification Thiosulfate Sulfurtransferase Molecular Weight Biochemistry Sulfurtransferases Density gradient ultracentrifugation Acinetobacter calcoaceticus Thiosulfate sulfurtransferase |
Zdroj: | Canadian Journal of Microbiology. 26:281-286 |
ISSN: | 1480-3275 0008-4166 |
DOI: | 10.1139/m80-047 |
Popis: | Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was found to be constitutively present as an intracellular enzyme in Acinetobacter calcoaceticus. The soluble enzyme was purified 40.9-fold by a procedure which included ultracentrifugation, ethanol precipitation, CM-Sephadex batchwise separation, QAE-50 ion exchange chromatography, and sucrose density gradient ultracentrifugation. The enzyme had a molecular weight of approximately 35 000 with a pH optimum of 8–8.5. Activity was substantially enhanced by supplements of 2-mercaptoethanol and to a lesser extent by cysteine–HCl or reduced glutathione. No degradation of the enzyme into smaller subunits was observed when treated with 2-mercaptoethanol. |
Databáze: | OpenAIRE |
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