Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides
| Autor: | Marcel Hövels, Uwe Deppenmeier, Konrad Kosciow |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Sucrose
Glycoside Hydrolases Polymers and Plastics Levanase Gluconobacter medicine.medical_treatment Gene Expression Oligosaccharides Fructose 02 engineering and technology Disaccharides 010402 general chemistry medicine.disease_cause 01 natural sciences Hydrolysis chemistry.chemical_compound Bacterial Proteins Escherichia coli Materials Chemistry medicine Humans Incubation Chemistry Prebiotic Organic Chemistry Levansucrase 021001 nanoscience & nanotechnology Recombinant Proteins Fructans 0104 chemical sciences Prebiotics Hexosyltransferases Biochemistry Azotobacter Phleum Azotobacter chroococcum 0210 nano-technology |
| Zdroj: | Carbohydrate Polymers. 255:117384 |
| ISSN: | 0144-8617 |
| Popis: | Prebiotics are known for their ability to modulate the composition of the human microbiome and mediate health-promoting benefits. Endo-levanases, which hydrolyze levan into short-chain FOS, could be used for the production of levan-based prebiotics. The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties. Starting from levan isolated from Timothy grass, LevB2286 produced FOS ranging from DP 2 – 8. In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation. The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose. 387.4 ± 17.3 g L−1 FOS were produced within 48 h by the production strategy based on crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously. |
| Databáze: | OpenAIRE |
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