Effect of pharmacological doses of ascorbic acid on the hepatic microsomal haemoproteins in the guinea-pig
| Autor: | Judith L. Sutton, J. W. T. Dickerson, Tapan K. Basu |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Hemeproteins
Male Vitamin Guinea Pigs Medicine (miscellaneous) Endogeny Ascorbic Acid Pharmacology Cofactor Guinea pig chemistry.chemical_compound Cytochrome P-450 Enzyme System Animals Ascorbic Acid Deficiency Nutrition and Dietetics Dose-Response Relationship Drug biology Cholesterol Cytochrome b Group Ascorbic acid Cytochromes b5 Biochemistry chemistry Microsomes Liver Microsome biology.protein |
| Zdroj: | British Journal of Nutrition. 49:27-33 |
| ISSN: | 1475-2662 0007-1145 |
| Popis: | 1. Ascorbic acid deficiency results in a reduction in the activity of the hepatic mixed function oxidase systems in the guinea-pig. In this study, male Dunkin-Hartley guinea-pigs were given 0, 50, 100, 200 or 300 mg ascorbic acid/d in two equal doses in buffered sucrose solution (200 g/l) for 4 d. Controls received an equal volume of sucrose solution.2. A dose of 50 mg ascorbic acid/d resulted in a significant rise in the specific activities of both cytochromes P-450 and b5. At doses of 200 and 300 mg ascorbic acid/d the concentration of both haemoproteins was significantly lower than the control values. These effects were mirrored by total microsomal haem concentration.3. These results suggest that when given in large doses, ascorbic acid ceases to act simply as a vitamin and should be considered a drug competing for substrates and cofactors with co-administered drugs and endogenous substrates such as cholesterol. |
| Databáze: | OpenAIRE |
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