A dry extract of Phyllanthus niruri protects normal cells and induces apoptosis in human liver carcinoma cells
| Autor: | Júlia Glória Lucatelli Pires, Helainy Daline Oliveira Mâcedo, Raimundo Fernandes de Araújo Júnior, Tatiane Pereira de Souza, Luiz Alberto Lira Soares, Ana Luiza C. de S. L. Oliveira, Aurigena Antunes de Araújo, Gerlane Coelho Bernardo Guerra, Pedro Ros Petrovick |
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| Rok vydání: | 2012 |
| Předmět: |
Programmed cell death
Carcinoma Hepatocellular Phyllanthus Cell Survival Apoptosis Pilot Projects Pharmacology Biology General Biochemistry Genetics and Molecular Biology HT29 Cells Cell Line Tumor Humans Cytotoxic T cell Caspase 3 Plant Extracts Liver Neoplasms Hep G2 Cells biology.organism_classification Antineoplastic Agents Phytogenic HaCaT Cell culture Cancer cell Immunology |
| Zdroj: | Experimental Biology and Medicine. 237:1281-1288 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.1258/ebm.2012.012130 |
| Popis: | The ability to induce apoptosis is an important marker for cytotoxic antitumor agents. Some natural compounds have been shown to modulate apoptosis pathways that are frequently blocked in human cancers, and therefore, these compounds provide novel opportunities for cancer drug development. Phyllanthus, a plant genus of the family Euphorbiaceae, exhibits multiple pharmacological actions. Of these, Phyllanthus niruri extracts exhibit significant antitumor activity, which is consistent with the traditional medicinal use of this plant. To examine the apoptotic effects of a spray-dried extract of P. niruri (SDEPN), human hepatocellular carcinoma cells (HepG2, Huh-7), colorectal carcinoma cells (Ht29) and keratinocytes (HaCaT) were exposed to the extract for 4, 8 and 24 h. Flow cytometry and caspase-3 immunostaining were used to detect apoptosis, while analysis of variance was applied to identify significant differences between groups ( P < 0.05). At all timepoints, the SDEPN induced significantly different cytotoxic effects for HepG2 and Huh-7 cells compared with control cells ( P < 0.001). In contrast, the SDEPN had a protective effect on HaCaT cells compared with control cells at all timepoints ( P < 0.001). In caspase-3 assays, activation was detected after cell death was induced in Huh-7 and HepG2 cancer cells by the SDEPN. In combination, these results indicate that the SDEPN is selectively toxic towards cancer cell lines, yet is protective towards normal cells. |
| Databáze: | OpenAIRE |
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