After Experimental Trypanosoma cruzi Infection, Dying Hepatic CD3+TCRαβ+B220+ T Lymphocytes Are Rescued from Death by Peripheral T Cells and Become Activated
| Autor: | Marcos Meuser Batista, Otacilio C. Moreira, Fernando Freitas, Andrea Henriques-Pons, Cynthia Machado Cascabulho, Juliana Rodrigues Guimarães, Marcelo Meuser-Batista, Samuel Iwao Horita, Natalia Vacani-Martins, Daniela Gois Beghini |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
CD3 Population lcsh:Medicine chemical and pharmacologic phenomena liver immune response CD11a CD49d Article Trypanosoma cruzi infection immune system diseases TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY hemic and lymphatic diseases parasitic diseases CD3+B220+ T lymphocytes Immunology and Allergy Cytotoxic T cell education hepatic T lymphocytes Molecular Biology education.field_of_study General Immunology and Microbiology biology Chemistry lcsh:R CD44 hemic and immune systems T lymphocyte Molecular biology cell death Infectious Diseases Apoptosis biology.protein |
| Zdroj: | Pathogens Volume 9 Issue 9 Pathogens, Vol 9, Iss 717, p 717 (2020) |
| ISSN: | 2076-0817 |
| Popis: | The unusual phenotype of CD3+ T lymphocyte expressing B220, a marker originally attributed to B lymphocytes, was first observed in the liver of Fas/Fas-L-deficient mice as a marker of apoptotic T lymphocytes. However, other CD3+B220+ T lymphocyte populations were later described in the periphery as functional cytotoxic or regulatory cells, for example. Then, in this work, we studied whether hepatic CD3+B220+ T lymphocytes could play a role in experimental Trypanosoma cruzi infection. In control and infected mice, we observed two subpopulations that could be discerned based on CD117 expression, which were conventional apoptotic CD3+B220+(CD117−) and thymus-independent CD3+B220+CD117+ T lymphocytes. Regardless of CD117 expression, most B220+ T lymphocytes were 7AAD+, confirming this molecule as a marker of dying T cells. However, after infection, we found that around 15% of the CD3+B220+CD117+ hepatic population became B220 and 7AAD negative, turned into CD90.2+, and upregulated the expression of CD44, CD49d, and CD11a, a phenotype consistent with activated T lymphocytes. Moreover, we observed that the hepatic CD3+B220+CD117+ population was rescued from death by previously activated peripheral T lymphocytes. Our results extend the comprehension of the hepatic CD3+B220+ T lymphocyte subpopulations and illustrate the complex interactions that occur in the liver. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|