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Comparison of deduced amino acid sequences between PyDes6 of Pythium sp. BCC53698 and other ∆6-desaturases, including P. infestans (PinDES6), P. splendens (PspDES6), N. oculata (NoDES6), M. alpina (MaDES6), M. rouxii (MrDES6) and Borage officinalis (BoDES6). The conserved histidine boxes and cytochrome b 5 heme-binding motif are underlined and boxed, respectively. Figure S2. Phylogenetic relationships between PyDes6 (Pythium sp. BCC53698) and ∆6-desaturase genes of other organisms. Bootstrap values from 1000 replicates test are shown at individual nodes. Abbreviations of other desaturase genes in individual organisms: PciDES6, P. citrophthora; PsoDES6, P.sojae; PinDES6, P. infestans; PirDES6, P. irregular; PspDES6, P. splendens; AlDES6, Albugo laibachii; NoDES6, N. oculata; EsDES6, Ectocarpus siliculosus; TpDES6, Thalassiosira pseudonana; PtDES6, P. tricornutum; MaDES6, M. alpina; RsDES6, Rhizopus stolonifer; McDES6, Mucor circinelloides; MrDES6, M. rouxii; MpDES6, Marchantia polymorpha; PpDES6, Physcomitrella patens; BoDES6, B. officinalis. Figure S3. Growth of the recombinant yeast strains carrying the empty vector (pYES2), PyDes6 and MPyDes6 genes. All strains were cultivated in SD medium containing 20 g/l of raffinose for 96 h. Cell growth is represented in terms of dry cell weight (grey bar) and cell density at OD600 (upward diagonals bar). (DOCX 1439 kb) |
